| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CDKN1A (1-159) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
Cdkn1a |
| Gene ID: |
12575 |
| Uniprot ID: |
CDN1A_MOUSE |
| Immunogen Region: |
1-159 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-159 of mouse CDKN1A (NP_031695.1). |
| Immunogen Sequence: |
MSNPGDVRPVPHRSKVCRCL FGPVDSEQLRRDCDALMAGC LQEARERWNFDFVTETPLEG NFVWERVRSLGLPKVYLSPG SRSRDDLGGDKRPSTSSALL QGPAPEDHVALSLSCTLVSE RPEDSPGGPGTSQGRKRRQT SLTDFYHSKRRLVFCKRKP |
| Post Translational Modifications | Phosphorylation of Thr-140 or Ser-141 impairs binding to PCNA. Phosphorylation at Ser-112 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex. Phosphorylation of Thr-140 by PIM2 enhances its stability and inhibits cell proliferation. Phosphorylation of Thr-140 by PIM1 results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. UV radiation-induced phosphorylation at Ser-78 and Ser-141 by NUAK1 leads to its degradation. Ubiquitinated by MKRN1.leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation. Ubiquitination at Ser-2 leads to degradation by the proteasome pathway. Ubiquitinated by RNF114.leading to proteasomal degradation. Acetylation leads to protein stability. Acetylated in vitro on Lys-136, Lys-149, Lys-156 and Lys-158. Deacetylation by HDAC1 is prevented by competitive binding of C10orf90/FATS to HDAC1. |
| Function | May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding. Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest. Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest. Involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Also involved in p53-independent DNA damage-induced G2 arrest mediated by CREB3L1 in astrocytes and osteoblasts. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding. |
| Protein Name | Cyclin-Dependent Kinase Inhibitor 1Cdk-Interacting Protein 1Melanoma Differentiation-Associated ProteinP21 |
| Database Links | Reactome: R-MMU-187577Reactome: -MMU-198323Reactome: -MMU-2559582Reactome: -MMU-2559586Reactome: -MMU-6804116Reactome: -MMU-69202Reactome: -MMU-69231Reactome: -MMU-69563Reactome: -MMU-69656Reactome: -MMU-8852276Reactome: -MMU-9616222 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-Cyclin-Dependent Kinase Inhibitor 1 antibodyAnti-Cdk-Interacting Protein 1 antibodyAnti-Melanoma Differentiation-Associated Protein antibodyAnti-P21 antibodyAnti-Cdkn1a antibodyAnti-Cip1 antibodyAnti-Waf1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
