• Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-CDK5RAP1 antibody (251-300 aa) (STJ92201)

SKU:
STJ92201

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Mitochondrial tRNA methylthiotransferase CDK5RAP1adenosine37 methylthiotransferase (251-300 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDK5RAP1
Gene ID: 51654
Uniprot ID: CK5P1_HUMAN
Immunogen Region: 251-300 aa
Specificity: CDK5 Activator-binding C42 Polyclonal Antibody detects endogenous levels of CDK5 Activator-binding C42 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human CDK5RAP1 at the amino acid range 251-300
Function Methylthiotransferase that catalyzes the conversion of N6-(dimethylallyl)adenosine (i(6)A) to 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 (adjacent to the 3'-end of the anticodon) of four mitochondrial DNA-encoded tRNAs (Ser(UCN), Phe, Tyr and Trp). Essential for efficient and highly accurate protein translation by the ribosome. Specifically inhibits CDK5 activation by CDK5R1. Essential for efficient mitochondrial protein synthesis and respiratory chain.shows pathological consequences in mitochondrial disease.
Protein Name Mitochondrial Trna Methylthiotransferase Cdk5rap1
Cdk5 Activator-Binding Protein C42
Cdk5 Regulatory Subunit-Associated Protein 1
Mt-Trna-2-Methylthio-N6-Dimethylallyladenosine Synthase
Mt-Trna-N6-(Dimethylallyladenosine(37 Methylthiotransferase
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Mitochondrial Trna Methylthiotransferase Cdk5rap1 antibody
Anti-Cdk5 Activator-Binding Protein C42 antibody
Anti-Cdk5 Regulatory Subunit-Associated Protein 1 antibody
Anti-Mt-Trna-2-Methylthio-N6-Dimethylallyladenosine Synthase antibody
Anti-Mt-Trna-N6-(Dimethylallyladenosine(37 Methylthiotransferase antibody
Anti-CDK5RAP1 antibody
Anti-C20orf34 antibody
Anti-CGI-05 antibody
Anti-HSPC167 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance