• Western blot analysis of lysates from rat brain cells, using CDK5R1 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of HeLa cells, using CDK5R1 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using p35 Polyclonal Antibody diluted at 1：1000
  • Western blot analysis of HELA cells using p35 Polyclonal Antibody diluted at 1：1000
  • Immunofluorescence analysis of Hela cell. 1, p35 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase 9 monoclonal antibody (3-20) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-CDK5R1 antibody (11-60 aa) (STJ94875)

SKU:
STJ94875

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Cyclin-dependent kinase 5 activator 1 (11-60 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDK5R1
Gene ID: 8851
Uniprot ID: CD5R1_HUMAN
Immunogen Region: 11-60 aa
Specificity: p35 Polyclonal Antibody detects endogenous levels of p35 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human CDK5R1 at the amino acid range 11-60
Post Translational Modifications The p35 form is proteolytically cleaved by calpain, giving rise to the p25 form. P35 has a 5 to 10 fold shorter half-life compared to p25. The conversion results in deregulation of the CDK5 kinase: p25/CDK5 kinase displays an increased and altered tau phosphorylation in comparison to the p35/CDK5 kinase in vivo. Myristoylated. A proper myristoylation signal is essential for the proper distribution of p35. Ubiquitinated, leading to its degradation: degradation of p35 by proteasome results in down-regulation of CDK5 activity. During this process, CDK5 phosphorylates p35 and induces its ubiquitination and subsequent degradation. Ubiquitinated by the CRL2(FEM1B) complex, which recognizes the -Gly-Leu-Asp-Arg C-degron at the C-terminus, leading to its degradation. Phosphorylation at Ser-8 and Thr-138 by CDK5 prevents calpain-mediated proteolysis.
Function p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Involved in dendritic spine morphogenesis by mediating the EFNA1-EPHA4 signaling. Activator of TPKII. The complex p35/CDK5 participates in the regulation of the circadian clock by modulating the function of CLOCK protein: phosphorylates CLOCK at 'Thr-451' and 'Thr-461' and regulates the transcriptional activity of the CLOCK-BMAL1 heterodimer in association with altered stability and subcellular distribution.
Protein Name Cyclin-Dependent Kinase 5 Activator 1
Cdk5 Activator 1
Cyclin-Dependent Kinase 5 Regulatory Subunit 1
Tpkii Regulatory Subunit Cleaved Into - Cyclin-Dependent Kinase 5 Activator 1 - P35
P35 - Cyclin-Dependent Kinase 5 Activator 1 - P25
P25
Tau Protein Kinase Ii 23 Kda Subunit
P23
Database Links Reactome: R-HSA-399956
Reactome: R-HSA-6804756
Reactome: R-HSA-8862803
Reactome: R-HSA-9031628
Reactome: R-HSA-9032845
Reactome: R-HSA-9768919
Cellular Localisation Cyclin-Dependent Kinase 5 Activator 1
P35: Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Cell Projection
Neuron Projection
In The Primary Cortical Neurons
P35 Is Present In The Peripheries And Nerve Terminals
P25: Nucleus
Cytoplasm
Perinuclear Region
Perikaryon
The Conversion Of P35 To P25 Relocalizes The Protein From The Cell Periphery To The Cytoplasm
In Nuclear And Perinuclear Regions
P25 Is Primarily Concentrated In The Cell Soma And Is Largely Absent From Neurites
Alternative Antibody Names Anti-Cyclin-Dependent Kinase 5 Activator 1 antibody
Anti-Cdk5 Activator 1 antibody
Anti-Cyclin-Dependent Kinase 5 Regulatory Subunit 1 antibody
Anti-Tpkii Regulatory Subunit Cleaved Into - Cyclin-Dependent Kinase 5 Activator 1 - P35 antibody
Anti-P35 - Cyclin-Dependent Kinase 5 Activator 1 - P25 antibody
Anti-P25 antibody
Anti-Tau Protein Kinase Ii 23 Kda Subunit antibody
Anti-P23 antibody
Anti-CDK5R1 antibody
Anti-CDK5R antibody
Anti-NCK5A antibody

Information sourced from Uniprot.org

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