• Western blot analysis of various lysates, using CDK4 rabbit monoclonal antibody (STJ11101279) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts from wild type (WT) and CDK4 rabbit monoclonal antibody knockout (KO) HeLa (KO) cells, using CDK4 rabbit monoclonal antibody (STJ11101279) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded mouse lung using CDK4 rabbit monoclonal antibody (STJ11101279) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using CDK4 rabbit monoclonal antibody (STJ11101279) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat lung using CDK4 rabbit monoclonal antibody (STJ11101279) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat ovary using CDK4 rabbit monoclonal antibody (STJ11101279) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of CDK4 in paraffin-embedded human cervix cancer tissue using [KO Validated] CDK4 rabbit monoclonal antibody (STJ11101279) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of CDK4 in paraffin-embedded human tonsil tissue using [KO Validated] CDK4 rabbit monoclonal antibody (STJ11101279) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of CDK4 in paraffin-embedded human breast cancer tissue using [KO Validated] CDK4 rabbit monoclonal antibody (STJ11101279) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of CDK4 in paraffin-embedded human cervix cancer tissue using [KO Validated] CDK4 rabbit monoclonal antibody (STJ11101279) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of CDK4 in paraffin-embedded human prostate tissue using [KO Validated] CDK4 rabbit monoclonal antibody (STJ11101279) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-CDK4 antibody (204-303) [S9MR] (STJ11101279)

SKU:
STJ11101279

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CDK4 (204-303) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDK4
Gene ID: 1019
Uniprot ID: CDK4_HUMAN
Immunogen Region: 204-303
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 204-303 of human CDK4 (NP_000066.1).
Immunogen Sequence: FAEMFRRKPLFCGNSEADQL GKIFDLIGLPPEDDWPRDVS LPRGAFPPRGPRPVQSVVPE MEESGAQLLLEMLTFNPHKR ISAFRALQHSYLHKDEGNPE
Post Translational Modifications Phosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B.
Function Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Protein Name Cyclin-Dependent Kinase 4
Cell Division Protein Kinase 4
Psk-J3
Database Links Reactome: R-HSA-187577
Reactome: R-HSA-2559580
Reactome: R-HSA-2559582
Reactome: R-HSA-2559585
Reactome: R-HSA-3214858
Reactome: R-HSA-381340
Reactome: R-HSA-69231
Reactome: R-HSA-75815
Reactome: R-HSA-8849470
Reactome: R-HSA-8878166
Reactome: R-HSA-912446
Reactome: R-HSA-9616222
Reactome: R-HSA-9630791
Reactome: R-HSA-9630794
Reactome: R-HSA-9632697
Reactome: R-HSA-9632700
Reactome: R-HSA-9661069
Reactome: R-HSA-9754119
Cellular Localisation Cytoplasm
Nucleus
Nucleus Membrane
Cytoplasmic When Non-Complexed
Forms A Cyclin D-Cdk4 Complex In The Cytoplasm As Cells Progress Through G(1) Phase
The Complex Accumulates On The Nuclear Membrane And Enters The Nucleus On Transition From G(1) To S Phase
Also Present In Nucleoli And Heterochromatin Lumps
Colocalizes With Rb1 After Release Into The Nucleus
Alternative Antibody Names Anti-Cyclin-Dependent Kinase 4 antibody
Anti-Cell Division Protein Kinase 4 antibody
Anti-Psk-J3 antibody
Anti-CDK4 antibody

Information sourced from Uniprot.org

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