![Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] CDK4 Rabbit polyclonal antibody (STJ11104280) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/176924/367965/STJ11104280_2__07506.1713133497.jpg?c=1 "Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] CDK4 Rabbit polyclonal antibody (STJ11104280) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunohistochemistry analysis of CDK4 in paraffin-embedded human colon using [KO Validated] CDK4 Rabbit polyclonal antibody (STJ11104280) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/176924/367966/STJ11104280_3__54231.1713133498.jpg?c=1 "Immunohistochemistry analysis of CDK4 in paraffin-embedded human colon using [KO Validated] CDK4 Rabbit polyclonal antibody (STJ11104280) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
![Western blot analysis of lysates from wild type (WT) and CDK4 knockout (KO) HeLa cells, using [KO Validated] CDK4 Rabbit polyclonal antibody (STJ11104280) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/176924/367967/STJ11104280_4__69620.1713133499.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CDK4 knockout (KO) HeLa cells, using [KO Validated] CDK4 Rabbit polyclonal antibody (STJ11104280) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western blot analysis of lysates from 293T cells, using [KO Validated] CDK4 Rabbit polyclonal antibody (STJ11104280) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/176924/367968/STJ11104280_5__10220.1713133500.jpg?c=1 "Western blot analysis of lysates from 293T cells, using [KO Validated] CDK4 Rabbit polyclonal antibody (STJ11104280) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western blot analysis of various lysates using [KO Validated] CDK4 Rabbit polyclonal antibody (STJ11104280) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/176924/367969/STJ11104280_6__54093.1713133501.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] CDK4 Rabbit polyclonal antibody (STJ11104280) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/IP/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IHC-P:1:50-1:100 IF/ICC:1:50-1:200 IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CDK4 |
| Gene ID: | 1019 |
| Uniprot ID: | CDK4_HUMAN |
| Immunogen Region: | 200-303 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 200-303 of human CDK4 (NP_000066.1). |
| Immunogen Sequence: | VGCIFAEMFRRKPLFCGNSE ADQLGKIFDLIGLPPEDDWP RDVSLPRGAFPPRGPRPVQS VVPEMEESGAQLLLEMLTFN PHKRISAFRALQHSYLHKDE GNPE |
| Post Translational Modifications | Phosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B. |
| Function | Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. |
| Protein Name | Cyclin-Dependent Kinase 4 Cell Division Protein Kinase 4 Psk-J3 |
| Database Links | Reactome: R-HSA-187577 Reactome: R-HSA-2559580 Reactome: R-HSA-2559582 Reactome: R-HSA-2559585 Reactome: R-HSA-3214858 Reactome: R-HSA-381340 Reactome: R-HSA-69231 Reactome: R-HSA-75815 Reactome: R-HSA-8849470 Reactome: R-HSA-8878166 Reactome: R-HSA-912446 Reactome: R-HSA-9616222 Reactome: R-HSA-9630791 Reactome: R-HSA-9630794 Reactome: R-HSA-9632697 Reactome: R-HSA-9632700 Reactome: R-HSA-9661069 Reactome: R-HSA-9754119 |
| Cellular Localisation | Cytoplasm Nucleus Nucleus Membrane Cytoplasmic When Non-Complexed Forms A Cyclin D-Cdk4 Complex In The Cytoplasm As Cells Progress Through G(1) Phase The Complex Accumulates On The Nuclear Membrane And Enters The Nucleus On Transition From G(1) To S Phase Also Present In Nucleoli And Heterochromatin Lumps Colocalizes With Rb1 After Release Into The Nucleus |
| Alternative Antibody Names | Anti-Cyclin-Dependent Kinase 4 antibody Anti-Cell Division Protein Kinase 4 antibody Anti-Psk-J3 antibody Anti-CDK4 antibody |
Information sourced from Uniprot.org