![Western blot analysis of lysates from wild type (WT) and CDK2AP1 knockout (KO) 293T cells, using [KO Validated] CDK2AP1 Rabbit polyclonal antibody (STJ11100861) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89795/358264/STJ11100861_1__24480.1713122604.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CDK2AP1 knockout (KO) 293T cells, using [KO Validated] CDK2AP1 Rabbit polyclonal antibody (STJ11100861) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western blot analysis of lysates from wild type (WT) and CDK2AP1 knockout (KO) 293T cells, using [KO Validated] CDK2AP1 Rabbit polyclonal antibody (STJ11100861) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89795/358264/STJ11100861_1__24480.1713122604.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CDK2AP1 knockout (KO) 293T cells, using [KO Validated] CDK2AP1 Rabbit polyclonal antibody (STJ11100861) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CDK2AP1 |
| Gene ID: | 8099 |
| Uniprot ID: | CDKA1_HUMAN |
| Immunogen Region: | 1-115 |
| Immunogen Sequence: | MSYKPNLAAHMPAAALNAAG SVHSPSTSMATSSQYRQLLS DYGPPSLGYTQGTGNSQVPQ SKYAELLAIIEELGKEIRPT YAGSKSAMERLKRGIIHARG LVRECLAETERNARS |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-115 of human CDK2AP1 (NP_004633.1). |
| Post Translational Modifications | Phosphorylated in vitro by IKBKE at Ser-46. |
| Function | Inhibitor of cyclin-dependent kinase CDK2. Also acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. |
| Protein Name | Cyclin-Dependent Kinase 2-Associated Protein 1Cdk2-Associated Protein 1Deleted In Oral Cancer 1Doc-1Putative Oral Cancer Suppressor |
| Database Links | |
| Cellular Localisation | NucleusChromosome |
| Alternative Antibody Names | Anti-Cyclin-Dependent Kinase 2-Associated Protein 1 antibodyAnti-Cdk2-Associated Protein 1 antibodyAnti-Deleted In Oral Cancer 1 antibodyAnti-Doc-1 antibodyAnti-Putative Oral Cancer Suppressor antibodyAnti-CDK2AP1 antibodyAnti-CDKAP1 antibodyAnti-DOC1 antibody |
Information sourced from Uniprot.org
