• Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MRCKB Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from COLO cells, using MRCKB Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using MRCK Beta Polyclonal Antibody

Anti-CDC42BPB antibody (1641-1690) (STJ94193)

SKU:
STJ94193

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Serine/threonine-protein kinase MRCK beta (1641-1690) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDC42BPB
Gene ID: 9578
Uniprot ID: MRCKB_HUMAN
Immunogen Region: 1641-1690
Specificity: MRCK Beta Polyclonal Antibody detects endogenous levels of MRCK Beta protein.
Immunogen: The antiserum was produced against synthesized peptide derived from human MRCKB. AA range:1641-1690
Tissue Specificity Expressed in all tissues examined, with high levels in heart, brain, placenta and lung.
Post Translational Modifications Proteolytically cleaved by caspases upon apoptosis induction.
Function Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates PPP1R12A. In concert with FAM89B/LRAP25 mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation.
Protein Name Serine/Threonine-Protein Kinase Mrck Beta
Cdc42-Binding Protein Kinase Beta
Cdc42bp-Beta
Dmpk-Like Beta
Myotonic Dystrophy Kinase-Related Cdc42-Binding Kinase Beta
Mrck Beta
Myotonic Dystrophy Protein Kinase-Like Beta
Database Links Reactome: R-HSA-9013148
Reactome: R-HSA-9013406
Reactome: R-HSA-9013409
Cellular Localisation Cytoplasm
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cell Junction
Cell Projection
Lamellipodium
Displays A Dispersed Punctate Distribution And Concentrates Along The Cell Periphery
Especially At The Leading Edge And Cell-Cell Junction
This Concentration Is Ph-Domain Dependent
Detected At The Leading Edge Of Migrating Cells
Localization At The Leading Edge Of Migrating Cells Requires Interaction With Catalytically Active Cdc42
Localizes In The Lamellipodium In A Fam89b/Lrap25-Dependent Manner
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Mrck Beta antibody
Anti-Cdc42-Binding Protein Kinase Beta antibody
Anti-Cdc42bp-Beta antibody
Anti-Dmpk-Like Beta antibody
Anti-Myotonic Dystrophy Kinase-Related Cdc42-Binding Kinase Beta antibody
Anti-Mrck Beta antibody
Anti-Myotonic Dystrophy Protein Kinase-Like Beta antibody
Anti-CDC42BPB antibody
Anti-KIAA1124 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance