• Western blot analysis of lysate from K562 cells, using CDC34 Antibody.
  • Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:100
  • Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:100
  • Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:100
  • Western blot analysis of K562, PC-3, mouse liver cells using Cdc34 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-CDC34 antibody (121-170 aa) (STJ96554)

SKU:
STJ96554

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Ubiquitin-conjugating enzyme E2 R1 E2 ubiquitin-conjugating enzyme R1 (121-170 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDC34
Gene ID: 997
Uniprot ID: UB2R1_HUMAN
Immunogen Region: 121-170 aa
Specificity: Cdc34 Polyclonal Antibody detects endogenous levels of Cdc34 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human CDC34 at the amino acid range 121-170
Post Translational Modifications Autoubiquitinated. Autoubiquitination is promoted by the human herpes virus 1 protein ICP0 and leads to degradation by the Ubiquitin-proteasomal pathway. Phosphorylated by CK2. Phosphorylation of the C-terminal tail by CK2 controls the nuclear localization.
Function Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes.
Protein Name Ubiquitin-Conjugating Enzyme E2 R1
(E3-Independent E2 Ubiquitin-Conjugating Enzyme R1
E2 Ubiquitin-Conjugating Enzyme R1
Ubiquitin-Conjugating Enzyme E2-32 Kda Complementing
Ubiquitin-Conjugating Enzyme E2-Cdc34
Ubiquitin-Protein Ligase R1
Database Links Reactome: R-HSA-202424
Reactome: R-HSA-2871837
Reactome: R-HSA-5607764
Reactome: R-HSA-8866652
Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Nucleus
The Phosphorylation Of The C-Terminal Tail Plays An Important Role In Mediating Nuclear Localization
Colocalizes With Beta-Tubulin On Mitotic Spindles In Anaphase
Alternative Antibody Names Anti-Ubiquitin-Conjugating Enzyme E2 R1 antibody
Anti-(E3-Independent E2 Ubiquitin-Conjugating Enzyme R1 antibody
Anti-E2 Ubiquitin-Conjugating Enzyme R1 antibody
Anti-Ubiquitin-Conjugating Enzyme E2-32 Kda Complementing antibody
Anti-Ubiquitin-Conjugating Enzyme E2-Cdc34 antibody
Anti-Ubiquitin-Protein Ligase R1 antibody
Anti-CDC34 antibody
Anti-UBCH3 antibody
Anti-UBE2R1 antibody

Information sourced from Uniprot.org

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