| Host: | Mouse |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-Cell division cycle protein 27 homolog is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Monoclonal |
| Clone ID : | 5C12 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Formulation: | Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol. |
| Purification: | Affinity purification |
| Dilution Range: | WB 1:500-1:2000 IHC 1:200-1:1000 ELISA 1:10000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CDC27 |
| Gene ID: | 996 |
| Uniprot ID: | CDC27_HUMAN |
| Specificity: | CDC27 Monoclonal Antibody detects endogenous levels of CDC27 protein. |
| Immunogen: | Purified recombinant fragment of human CDC27 expressed in E. Coli. |
| Function | Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins. |
| Protein Name | Cell Division Cycle Protein 27 Homolog Anaphase-Promoting Complex Subunit 3 Apc3 Cdc27 Homolog Cdc27hs H-Nuc |
| Database Links | Reactome: R-HSA-141430 Reactome: R-HSA-174048 Reactome: R-HSA-174084 Reactome: R-HSA-174154 Reactome: R-HSA-174178 Reactome: R-HSA-174184 Reactome: R-HSA-176407 Reactome: R-HSA-176408 Reactome: R-HSA-176409 Reactome: R-HSA-176412 Reactome: R-HSA-179409 Reactome: R-HSA-2467813 Reactome: R-HSA-2559582 Reactome: R-HSA-68867 Reactome: R-HSA-69017 Reactome: R-HSA-8853884 Reactome: R-HSA-9687136 Reactome: R-HSA-983168 |
| Cellular Localisation | Nucleus Cytoplasm Cytoskeleton Spindle |
| Alternative Antibody Names | Anti-Cell Division Cycle Protein 27 Homolog antibody Anti-Anaphase-Promoting Complex Subunit 3 antibody Anti-Apc3 antibody Anti-Cdc27 Homolog antibody Anti-Cdc27hs antibody Anti-H-Nuc antibody Anti-CDC27 antibody Anti-ANAPC3 antibody Anti-D0S1430E antibody Anti-D17S978E antibody |
Information sourced from Uniprot.org
