• Immunohistochemistry analysis of CDC23 in paraffin-embedded mouse lung using CDC23 Rabbit polyclonal antibody (STJ117310) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of CDC23 in paraffin-embedded human tonsil using CDC23 Rabbit polyclonal antibody (STJ117310) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of CDC23 in paraffin-embedded rat spleen using CDC23 Rabbit polyclonal antibody (STJ117310) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using CDC23 Rabbit polyclonal antibody (STJ117310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CDC23 antibody (50-150) (STJ117310)

SKU:
STJ117310

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CDC23 (50-150) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDC23
Gene ID: 8697
Uniprot ID: CDC23_HUMAN
Immunogen Region: 50-150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human CDC23 (NP_004652.2).
Immunogen Sequence: HSSKWSAELAFSLPALPLAE LQPPPPITEEDAQDMDAYTL AKAYFDVKEYDRAAHFLHGC NSKKAYFLYMYSRYLSGEKK KDDETVDSLGPLEKGQVKNE A
Post Translational Modifications Phosphorylated. Phosphorylation on Thr-562 occurs specifically during mitosis.
Function Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
Protein Name Cell Division Cycle Protein 23 Homolog
Anaphase-Promoting Complex Subunit 8
Apc8
Cyclosome Subunit 8
Database Links Reactome: R-HSA-141430
Reactome: R-HSA-174048
Reactome: R-HSA-174084
Reactome: R-HSA-174154
Reactome: R-HSA-174178
Reactome: R-HSA-174184
Reactome: R-HSA-176407
Reactome: R-HSA-176408
Reactome: R-HSA-176409
Reactome: R-HSA-176412
Reactome: R-HSA-179409
Reactome: R-HSA-2467813
Reactome: R-HSA-2559582
Reactome: R-HSA-68867
Reactome: R-HSA-69017
Reactome: R-HSA-8853884
Reactome: R-HSA-9687136
Reactome: R-HSA-983168
Alternative Antibody Names Anti-Cell Division Cycle Protein 23 Homolog antibody
Anti-Anaphase-Promoting Complex Subunit 8 antibody
Anti-Apc8 antibody
Anti-Cyclosome Subunit 8 antibody
Anti-CDC23 antibody
Anti-ANAPC8 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance