• Western blot analysis of various lysates, using CD99 Rabbit polyclonal antibody (STJ11105426) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC) :CHOExposure time: 90s.

Anti-CD99 antibody (23-124) (STJ11105426)

SKU:
STJ11105426

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Cd99 antigen (23-124) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD99
Gene ID: 4267
Uniprot ID: CD99_HUMAN
Immunogen Region: 23-124
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 23-124 of human CD99 (NP_002405.1).
Immunogen Sequence: DGGFDLSDALPDNENKKPTA IPKKPSAGDDFDLGDAVVDG ENDDPRPPNPPKPMPNPNPN HPSSSGSFSDADLADGVSGG EGKGGSDGGGSHRKEGEEAD AP
Post Translational Modifications Extensively O-glycosylated.
Function Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes.
Protein Name Cd99 Antigen
12e7
E2 Antigen
Protein Mic2
T-Cell Surface Glycoprotein E2
Cd Antigen Cd99
Database Links Reactome: R-HSA-198933
Reactome: R-HSA-202733
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Cd99 Antigen antibody
Anti-12e7 antibody
Anti-E2 Antigen antibody
Anti-Protein Mic2 antibody
Anti-T-Cell Surface Glycoprotein E2 antibody
Anti-Cd Antigen Cd99 antibody
Anti-CD99 antibody
Anti-MIC2 antibody
Anti-MIC2X antibody
Anti-MIC2Y antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance