• Various whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-CD8 a (ABT455) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: Daudi Lane 2: Raji Lane 3: MOLT-4Predicted band size: 26kDaObserved band size: 24kDa
  • Human appendix tissue was stained with Anti-CD8 (ABT304) Antibody
  • Human burkitt lymphoma tissue was stained with Anti-CD8 (ABT304) Antibody
  • Human lymphoma tissue was stained with Anti-CD8 (ABT304) Antibody
  • Human lymphoma tissue was stained with Anti-CD8 (ABT304) Antibody
  • Human tonsil tissue was stained with Anti-CD8 (ABT304) Antibody
  • Fluorescence multiplex immunohistochemical analysis of Human tonsil tissue (formalin-fixed paraffin-embedded section). Merged staining of Anti-Podoplanin (STJ197273), Anti-CD8 (STJ197218), Anti-MCM2. The immunostaining was performed on a Leica Biosystems BOND® MAX instrument with an Sextuple-Fluorescence kit. The section was incubated in 3 rounds of staining; sequentially for Anti-Podoplanin (STJ197273 1:200), Anti-CD8 (STJ197218 1:200), Anti-MCM2 (1:200). ; each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Leica Biosystems BOND® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (3D histech).
  • Fluorescence multiplex immunohistochemical analysis of Human tonsil tissue (formalin-fixed paraffin-embedded section). Merged staining of Anti-Podoplanin (STJ197273), Anti-CD8 (STJ197218), Anti-Ki-67 (STJ197092). The immunostaining was performed on a Leica Biosystems BOND® MAX instrument with an Sextuple-Fluorescence kit. The section was incubated in 3 rounds of staining; sequentially for Anti-Podoplanin (STJ197273 1:200), Anti-CD8 (STJ197218 1:200), Anti-Ki67 (STJ197092 1:200). ; each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Leica Biosystems BOND® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (3D histech).
  • Fluorescence multiplex immunohistochemical analysis of Human tonsil tissue (formalin-fixed paraffin-embedded section). Merged staining of Anti-Podoplanin (STJ197273), Anti-CD8 (STJ197218), Anti-MCM2. The immunostaining was performed on a Leica Biosystems BOND® MAX instrument with an Sextuple-Fluorescence kit. The section was incubated in 3 rounds of staining; sequentially for Anti-Podoplanin (STJ197273 1:200), Anti-CD8 (STJ197218 1:200), Anti-MCM2 (1:200). ; each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Leica Biosystems BOND® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (3D histech).

Anti-CD8A antibody (100-235 aa) [ABT304] (STJ197218)

SKU:
STJ197218

£168.00 - £226.00
Current Stock:
Host: Mouse
Applications: IHC/WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-T-cell surface glycoprotein CD8 alpha chain (100-235 aa) is suitable for use in Immunohistochemistry and Western Blot research applications.
Clonality: Monoclonal
Clone ID: ABT304
Conjugation: Unconjugated
Isotype: IgG2bk
Formulation: Liquid in PBS pH7.2, 0.03% Proclin 300, with stabilizing protein.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: IHC-P 1:200-400
WB 1:200-1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD8A
Gene ID: 925
Uniprot ID: CD8A_HUMAN
Immunogen Region: 100-235 aa
Specificity: The antibody can specifically recognize human CD8 protein, including two typies of dimer: Alpha Beta heterodimer or Alpha Alpha homodimer.
Immunogen: Synthesized peptide derived from the human CD8 at the amino acid range 100-235
Post Translational Modifications Palmitoylated, but association with CD8B seems to be more important for the enrichment of CD8A in lipid rafts. O-glycosylated. Phosphorylated in cytotoxic T-lymphocytes (CTLs) following activation.
Function Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
Protein Name T-Cell Surface Glycoprotein Cd8 Alpha Chain
T-Lymphocyte Differentiation Antigen T8/Leu-2
Cd Antigen Cd8a
Database Links Reactome: R-HSA-198933
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Cd8a Localizes To Lipid Rafts Only When Associated With Its Partner Cd8b
Isoform 2: Secreted
Alternative Antibody Names Anti-T-Cell Surface Glycoprotein Cd8 Alpha Chain antibody
Anti-T-Lymphocyte Differentiation Antigen T8/Leu-2 antibody
Anti-Cd Antigen Cd8a antibody
Anti-CD8A antibody
Anti-MAL antibody

Information sourced from Uniprot.org

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