| Host: |
Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CD82 (220-267 aa) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Lyophilised |
| Purification: |
IgG purified |
| Dilution Range: |
IHC, WB (confirmed by recombinant protein). A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
CD82 |
| Gene ID: |
3732 |
| Uniprot ID: |
CD82_HUMAN |
| Immunogen Region: |
220-267 aa |
| Specificity: |
Specific for CD82. |
| Immunogen: |
A synthetic peptide from aa region 220-267 of human CD82 conjugated to an immunogenic carrier protein was used as the antigen. The antigen shares 93% identity with rat and mouse sequences. |
| Post Translational Modifications | Palmitoylated. Palmitoylation contributes to oligomerization and surface expression. |
| Function | Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking. Acts as a attenuator of EGF signaling, facilitating ligand-induced endocytosis of the receptor and its subsequent desensitization. Mechanistically, modulates ligand-induced ubiquitination and trafficking of EGFR via E3 ligase CBL phosphorylation by PKC. Increases cell-matrix adhesion by regulating the membrane organization of integrin alpha4/ITA4. Modulates adhesion and suppresses cell migration through other integrins such as the alpha6/ITGA6 and beta1/ITGB1. Decreases cell-associated plasminogen activation by interfering with the interaction between urokinase-type plasminogen activator/PLAU and its receptor PLAUR. Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway. Plays a role in TLR9 trafficking to acidified CpG-containing compartments by controlling interaction between TLR9 and VAMP3 and subsequent myddosome assembly. Inhibits LPS-induced inflammatory response by preventing binding of LPS to TLR4 on the cell surface. Plays a role in the activation of macrophages into anti-inflammatory phenotypes. Independently of Toll-like receptor (TLR) signaling, is recruited to pathogen-containing phagosomes prior to fusion with lysosomes and thereby participates in antigen presentation. Acts also to control angiogenesis and switch angiogenic milieu to quiescent state by binding and sequestering VEGFA and PDGFB to inhibit the signaling they trigger via their respective cell surface receptor. |
| Protein Name | Cd82 AntigenC33 AntigenIa4Inducible Membrane Protein R2Metastasis Suppressor Kangai-1Suppressor Of Tumorigenicity 6 ProteinTetraspanin-27Tspan-27Cd Antigen Cd82 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinCytoplasmic VesiclePhagosome |
| Alternative Antibody Names | Anti-Cd82 Antigen antibodyAnti-C33 Antigen antibodyAnti-Ia4 antibodyAnti-Inducible Membrane Protein R2 antibodyAnti-Metastasis Suppressor Kangai-1 antibodyAnti-Suppressor Of Tumorigenicity 6 Protein antibodyAnti-Tetraspanin-27 antibodyAnti-Tspan-27 antibodyAnti-Cd Antigen Cd82 antibodyAnti-CD82 antibodyAnti-KAI1 antibodyAnti-SAR2 antibodyAnti-ST6 antibodyAnti-TSPAN27 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
