• Immunohistochemistry analysis of paraffin-embedded mouse brain using CD82 antibody (STJ28271) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human spleen using CD82 antibody (STJ28271) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using CD82 antibody (STJ28271) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of MCF7 cells, using CD82 antibody (STJ28271) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CD82 antibody (150-250) (STJ28271)

SKU:
STJ28271

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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:100-1:500
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD82
Gene ID: 3732
Uniprot ID: CD82_HUMAN
Immunogen Region: 150-250
Specificity: A synthetic peptide corresponding to a sequence within amino acids 150-250 of human CD82 (NP_002222.1).
Immunogen Sequence: CGWVSFYNWTDNAELMNRPE VTYPCSCEVKGEEDNSLSVR KGFCEAPGNRTQSGNHPEDW PVYQEGCMEKVQAWLQENLG IILGVGVGVAIIELLGMVLS I
Tissue Specificity Lymphoid specific.
Post Translational Modifications Palmitoylated. Palmitoylation contributes to oligomerization and surface expression.
Function Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking. Acts as a attenuator of EGF signaling, facilitating ligand-induced endocytosis of the receptor and its subsequent desensitization. Mechanistically, modulates ligand-induced ubiquitination and trafficking of EGFR via E3 ligase CBL phosphorylation by PKC. Increases cell-matrix adhesion by regulating the membrane organization of integrin alpha4/ITA4. Modulates adhesion and suppresses cell migration through other integrins such as the alpha6/ITGA6 and beta1/ITGB1. Decreases cell-associated plasminogen activation by interfering with the interaction between urokinase-type plasminogen activator/PLAU and its receptor PLAUR. Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway. Plays a role in TLR9 trafficking to acidified CpG-containing compartments by controlling interaction between TLR9 and VAMP3 and subsequent myddosome assembly. Inhibits LPS-induced inflammatory response by preventing binding of LPS to TLR4 on the cell surface. Plays a role in the activation of macrophages into anti-inflammatory phenotypes. Independently of Toll-like receptor (TLR) signaling, is recruited to pathogen-containing phagosomes prior to fusion with lysosomes and thereby participates in antigen presentation. Acts also to control angiogenesis and switch angiogenic milieu to quiescent state by binding and sequestering VEGFA and PDGFB to inhibit the signaling they trigger via their respective cell surface receptor.
Protein Name Cd82 Antigen
C33 Antigen
Ia4
Inducible Membrane Protein R2
Metastasis Suppressor Kangai-1
Suppressor Of Tumorigenicity 6 Protein
Tetraspanin-27
Tspan-27
Cd Antigen Cd82
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Cytoplasmic Vesicle
Phagosome
Alternative Antibody Names Anti-Cd82 Antigen antibody
Anti-C33 Antigen antibody
Anti-Ia4 antibody
Anti-Inducible Membrane Protein R2 antibody
Anti-Metastasis Suppressor Kangai-1 antibody
Anti-Suppressor Of Tumorigenicity 6 Protein antibody
Anti-Tetraspanin-27 antibody
Anti-Tspan-27 antibody
Anti-Cd Antigen Cd82 antibody
Anti-CD82 antibody
Anti-KAI1 antibody
Anti-SAR2 antibody
Anti-ST6 antibody
Anti-TSPAN27 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance