• Immunohistochemistry analysis of CD68. Sample: Human colon, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-mouse lgG, Ready to use, RT, 20min.
  • Immunohistochemistry analysis of CD68. Sample: Human liver, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-mouse lgG, Ready to use, RT, 20min.
  • Immunohistochemistry analysis of CD68. Sample: Human lung, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-mouse lgG, Ready to use, RT, 20min.
  • Immunohistochemistry analysis of CD68. Sample: Human renal cancer, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-mouse lgG, Ready to use, RT, 20min.
  • Immunohistochemistry analysis of CD68. Sample: Human tonsil, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-mouse lgG, Ready to use, RT, 20min.
  • Immunohistochemistry analysis of CD68. Sample: Human tonsil, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-mouse lgG, Ready to use, RT, 20min.
  • IF analysis of CD68red. Sample: Human colon, 4% PFA 12-24h. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:500, 4C overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG, 1:300, RT, 1h.
  • IF analysis of CD68red. Sample: Human gastric cancer, 4% PFA 12-24h. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:500, 4C overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG, 1:300, RT, 1h.
  • IF analysis of CD68red. Sample: Human liver cancer, 4% PFA 12-24h. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:500, 4C overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG, 1:300, RT, 1h.

Anti-CD68 antibody [SM0477] (STJA0010477)

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STJA0010477

£170.50 - £233.50
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Host: Mouse
Applications: IHC/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-CD68 is suitable for use in Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: SM0477
Conjugation: Unconjugated
Isotype: IgG1k
Formulation: PBS with 0.15% ProClin300, 100 Mu g/mL BSA and 50% glycerol.
Purification: Affinity purification
Dilution Range: IHC/IF (H) 1:500-1:1000
Storage Instruction: Store at-20C for up to one year, and avoid repeated freeze-thaw cycles.
Gene Symbol: CD68
Gene ID: 968
Uniprot ID: CD68_HUMAN
Immunogen: Recombinant protein corresponding to Human CD68
Post Translational Modifications N- and O-glycosylated.
Function Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
Protein Name Macrosialin
Gp110
Cd Antigen Cd68
Database Links Reactome: R-HSA-6798695
Cellular Localisation Isoform Short: Cell Membrane
Single-Pass Type I Membrane Protein
Isoform Long: Endosome Membrane
Lysosome Membrane
Alternative Antibody Names Anti-Macrosialin antibody
Anti-Gp110 antibody
Anti-Cd Antigen Cd68 antibody
Anti-CD68 antibody

Information sourced from Uniprot.org

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