| Short Description : | Mouse monoclonal antibody anti-CD59 is suitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation research applications. |
| Applications: | WB/ICC/IP |
| Host: | Mouse |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | M015 |
| Isotype: | IgG2b |
| Conjugation: | Unconjugated |
| Purification: | Protein G Purified |
| Dilution Range: | WB 1:1000ICC 1:100IP 1:100 |
| Formulation: | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CD59 |
| Gene ID: | 966 |
| Uniprot ID: | CD59_HUMAN |
| Immunogen: | Clone M015 was generated from a proprietary antigen related to the mature form of human CD59 from the A431 epidermoid carcinoma cell line. |
| Specificity: | Clone M015 detects 15-20 kDa* bands corresponding to CD59 on SDS-PAGE immunoblots of native human MDA-MB-231, A549, A431, and MCF7 cell lysates, as well as recombinant human CD59 protein. The antibody does not detect denatured CD59. The antibody can |
| Post Translational Modifications | N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine. Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants. Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes. |
| Function | Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. |
| Protein Name | Cd59 Glycoprotein1f5 Antigen20 Kda Homologous Restriction FactorHrf-20Hrf20Mac-Inhibitory ProteinMac-IpMem43 AntigenMembrane Attack Complex Inhibition FactorMacifMembrane Inhibitor Of Reactive LysisMirlProtectinCd Antigen Cd59 |
| Database Links | Reactome: R-HSA-204005Reactome: R-HSA-5694530Reactome: R-HSA-6798695Reactome: R-HSA-6807878Reactome: R-HSA-977606 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-AnchorSecretedSoluble Form Found In A Number Of Tissues |
| Alternative Antibody Names | Anti-Cd59 Glycoprotein antibodyAnti-1f5 Antigen antibodyAnti-20 Kda Homologous Restriction Factor antibodyAnti-Hrf-20 antibodyAnti-Hrf20 antibodyAnti-Mac-Inhibitory Protein antibodyAnti-Mac-Ip antibodyAnti-Mem43 Antigen antibodyAnti-Membrane Attack Complex Inhibition Factor antibodyAnti-Macif antibodyAnti-Membrane Inhibitor Of Reactive Lysis antibodyAnti-Mirl antibodyAnti-Protectin antibodyAnti-Cd Antigen Cd59 antibodyAnti-CD59 antibodyAnti-MIC11 antibodyAnti-MIN1 antibodyAnti-MIN2 antibodyAnti-MIN3 antibodyAnti-MSK21 antibody |
Information sourced from Uniprot.org
