• STJ72587 (0. 01µg/ml) staining of Human Frontal Cortex lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • STJ72587 Flow cytometric analysis of paraformaldehyde fixed U251 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-CD56/NCAM1-Isoform 2 antibody (Internal) (STJ72587)

SKU:
STJ72587-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC/FC
Reactivity: Human/Rat/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-CD56/NCAM1-Isoform 2 (Internal) is suitable for use in ELISA, Western Blot, Immunohistochemistry and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.01-0.03µg/ml
IHC-5µg/ml
FC-Flow cytometric analysis of U251 cells. 10ug/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: NCAM1
Gene ID: 4684
Uniprot ID: NCAM1_HUMAN
Immunogen Region: Internal
Accession Number: NP_851996.2
Specificity: This antibody is expected to recognize reported isoform 2 (NP_851996.2) only.
Immunogen Sequence: KASWTRPEKQETLD
Protein Name Neural Cell Adhesion Molecule 1
N-Cam-1
Ncam-1
Cd Antigen Cd56
Database Links Reactome: R-HSA-3000178
Reactome: R-HSA-375165
Reactome: R-HSA-419037
Reactome: R-HSA-445144
Reactome: R-HSA-5673001
Reactome: R-HSA-877300
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Isoform 2: Cell Membrane
Isoform 3: Cell Membrane
Lipid-Anchor
Gpi-Anchor
Isoform 4: Cell Membrane
Isoform 5: Secreted
Isoform 6: Secreted
Alternative Antibody Names Anti-Neural Cell Adhesion Molecule 1 antibody
Anti-N-Cam-1 antibody
Anti-Ncam-1 antibody
Anti-Cd Antigen Cd56 antibody
Anti-NCAM1 antibody
Anti-NCAM antibody

Information sourced from Uniprot.org

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