| Host: | Mouse |
| Applications: | FC/IF/IHC |
| Reactivity: | Human/Baboon |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-CD46 is suitable for use in Flow Cytometry, Immunofluorescence and Immunohistochemistry research applications. |
| Clonality : | Monoclonal |
| Clone ID : | EBS-CD-029 |
| Conjugation: | Unconjugated |
| Isotype: | IgG2ak |
| Formulation: | PBS with 0.02% Sodium Azide. |
| Purification: | Affinity purified from tissue culture. |
| Concentration: | Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Dilution Range: | Flow cytometry (0, 5-1, 0 µg/million cells in 0, 1 ml). Immunofluorescence (0, 5-1, 0 µg/ml). Immunohistology (1-2 µg/ml for 30 min at RT; an appropriate antigen retrieval method for staining of formalin-fixed tissues has not been established to date |
| Storage Instruction: | Store for up to 1 year at 2-8°C upon receipt. |
| Gene Symbol: | CD46 |
| Gene ID: | 4179 |
| Uniprot ID: | MCP_HUMAN |
| Immunogen: | A BALB/c mouse was immunized with stimulated human PBL and an EBV transformed human lymphoblastoid line. Fusion partner: NS-1. |
| Post Translational Modifications | N-glycosylated on Asn-83.Asn-114 and Asn-273 in most tissues, but probably less N-glycosylated in testis. N-glycosylation on Asn-114 and Asn-273 is required for cytoprotective function. N-glycosylation on Asn-114 is required for Measles virus binding. N-glycosylation on Asn-273 is required for Neisseria binding. N-glycosylation is not required for human adenovirus binding. Extensively O-glycosylated in the Ser/Thr-rich domain. O-glycosylation is required for Neisseria binding but not for Measles virus or human adenovirus binding. In epithelial cells, isoforms B/D/F/H/J/L/3 are phosphorylated by YES1 in response to infection by Neisseria gonorrhoeae.which promotes infectivity. In T-cells, these isoforms may be phosphorylated by LCK. |
| Function | Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. (Microbial infection) A number of viral and bacterial pathogens seem to bind MCP in order to exploit its immune regulation property and directly induce an immunosuppressive phenotype in T-cells. (Microbial infection) Acts as a receptor for Adenovirus subgroup B2 and Ad3. (Microbial infection) Acts as a receptor for cultured Measles virus. (Microbial infection) Acts as a receptor for Herpesvirus 6/HHV-6. (Microbial infection) May act as a receptor for pathogenic bacteria Neisseria and Streptococcus pyogenes. |
| Protein Name | Membrane Cofactor Protein Tlx Trophoblast Leukocyte Common Antigen Cd Antigen Cd46 |
| Database Links | Reactome: R-HSA-977606 |
| Cellular Localisation | Cytoplasmic Vesicle Secretory Vesicle Acrosome Inner Membrane Single-Pass Type I Membrane Protein Inner Acrosomal Membrane Of Spermatozoa Internalized Upon Binding Of Measles Virus Herpesvirus 6 Or Neisseria Gonorrhoeae Which Results In An Increased Susceptibility Of Infected Cells To Complement-Mediated Injury In Cancer Cells Or Cells Infected By Neisseria Shedding Leads To A Soluble Peptide |
| Alternative Antibody Names | Anti-Membrane Cofactor Protein antibody Anti-Tlx antibody Anti-Trophoblast Leukocyte Common Antigen antibody Anti-Cd Antigen Cd46 antibody Anti-CD46 antibody Anti-MCP antibody Anti-MIC10 antibody |
Information sourced from Uniprot.org