| Host: |
Mouse |
| Applications: |
FC/IF/IHC |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-CD46 is suitable for use in Flow Cytometry, Immunofluorescence and Immunohistochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
EBS-CD-028 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1k |
| Formulation: |
PBS with 0.02% Sodium Azide. |
| Purification: |
Affinity purified from tissue culture. |
| Concentration: |
Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Dilution Range: |
Flow cytometry (0, 5-1, 0 µg/million cells in 0, 1 ml). Immunofluorescence (0, 5-1, 0 µg/ml). Immunohistology (1-2 µg/ml for 30 min at RT; an appropriate antigen retrieval method for staining of formalin-fixed tissues has not been established to date |
| Storage Instruction: |
Store for up to 1 year at 2-8°C upon receipt. |
| Gene Symbol: |
CD46 |
| Gene ID: |
4179 |
| Uniprot ID: |
MCP_HUMAN |
| Immunogen: |
A BALB/c mouse was immunized with stimulated human PBL. Fusion partner: NS-1. |
| Tissue Specificity | Expressed by all cells except erythrocytes. |
| Post Translational Modifications | N-glycosylated on Asn-83.Asn-114 and Asn-273 in most tissues, but probably less N-glycosylated in testis. N-glycosylation on Asn-114 and Asn-273 is required for cytoprotective function. N-glycosylation on Asn-114 is required for Measles virus binding. N-glycosylation on Asn-273 is required for Neisseria binding. N-glycosylation is not required for human adenovirus binding. Extensively O-glycosylated in the Ser/Thr-rich domain. O-glycosylation is required for Neisseria binding but not for Measles virus or human adenovirus binding. In epithelial cells, isoforms B/D/F/H/J/L/3 are phosphorylated by YES1 in response to infection by Neisseria gonorrhoeae.which promotes infectivity. In T-cells, these isoforms may be phosphorylated by LCK. |
| Function | Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. (Microbial infection) A number of viral and bacterial pathogens seem to bind MCP in order to exploit its immune regulation property and directly induce an immunosuppressive phenotype in T-cells. (Microbial infection) Acts as a receptor for Adenovirus subgroup B2 and Ad3. (Microbial infection) Acts as a receptor for cultured Measles virus. (Microbial infection) Acts as a receptor for Herpesvirus 6/HHV-6. (Microbial infection) May act as a receptor for pathogenic bacteria Neisseria and Streptococcus pyogenes. |
| Protein Name | Membrane Cofactor ProteinTlxTrophoblast Leukocyte Common AntigenCd Antigen Cd46 |
| Database Links | Reactome: R-HSA-977606 |
| Cellular Localisation | Cytoplasmic VesicleSecretory VesicleAcrosome Inner MembraneSingle-Pass Type I Membrane ProteinInner Acrosomal Membrane Of SpermatozoaInternalized Upon Binding Of Measles VirusHerpesvirus 6 Or Neisseria GonorrhoeaeWhich Results In An Increased Susceptibility Of Infected Cells To Complement-Mediated InjuryIn Cancer Cells Or Cells Infected By NeisseriaShedding Leads To A Soluble Peptide |
| Alternative Antibody Names | Anti-Membrane Cofactor Protein antibodyAnti-Tlx antibodyAnti-Trophoblast Leukocyte Common Antigen antibodyAnti-Cd Antigen Cd46 antibodyAnti-CD46 antibodyAnti-MCP antibodyAnti-MIC10 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
