![C57BL/6 murine splenocytes were stained with Anti-CD45 antibody (STJA0000858) [Used at 0.2 ยตg/10<sup>6</sup> cells dilution] (filled gray histogram). Unstained splenocytes (empty black histogram) were used as control.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/145711/292369/STJA0000858_1__18746.1631206225.jpg?c=1 "C57BL/6 murine splenocytes were stained with Anti-CD45 antibody (STJA0000858) [Used at 0.2 ยตg/10<sup>6</sup> cells dilution] (filled gray histogram). Unstained splenocytes (empty black histogram) were used as control.")
![C57BL/6 murine splenocytes were stained with Anti-CD45 antibody (STJA0000858) [Used at 0.2 ยตg/10<sup>6</sup> cells dilution] (filled gray histogram). Unstained splenocytes (empty black histogram) were used as control.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/145711/292369/STJA0000858_1__18746.1631206225.jpg?c=1 "C57BL/6 murine splenocytes were stained with Anti-CD45 antibody (STJA0000858) [Used at 0.2 ยตg/10<sup>6</sup> cells dilution] (filled gray histogram). Unstained splenocytes (empty black histogram) were used as control.")
Anti-CD45/Ptprc antibody [30-F11] {PercP} (STJA0000858)
SPECIFICATIONS
ClonalityMonoclonal
HostRat
ConjugationPercP
IsotypeIgG2bk
General Information
| Short Description | Rat monoclonal anti-Receptor-type tyrosine-protein phosphatase C for use in FC in Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC |
| Host | Rat |
| Reactivity | Mouse |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 30-F11 |
| Isotype | IgG2bk |
| Conjugation | PercP |
| Concentration | 0.2 mg/mL |
| Formulation | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
| Storage Instruction | Store between 2-8ยฐC and protecting from prolonged exposure to light. Do not freeze this product. |
Target Information
| Gene Symbol | Ptprc |
| Gene ID | 19264 |
| Uniprot ID | PTPRC_MOUSE |
Additional Info
| Post Translational Modifications | Heavily N- and O-glycosylated. |
| Function | Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity. Interacts with CLEC10A at antigen presenting cell-T cell contact.CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity. |
| Protein Name | Receptor-Type Tyrosine-Protein Phosphatase CLeukocyte Common AntigenL-CaLymphocyte Antigen 5Ly-5T200Cd Antigen Cd45 |
| Database Links | Reactome: R-MMU-202427Reactome: -MMU-416700Reactome: -MMU-6798695 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinMembrane RaftSynapseColocalized With Dpp4 In Membrane Rafts |
| Alternative Antibody Names | Anti-Receptor-Type Tyrosine-Protein Phosphatase C antibodyAnti-Leukocyte Common Antigen antibodyAnti-L-Ca antibodyAnti-Lymphocyte Antigen 5 antibodyAnti-Ly-5 antibodyAnti-T200 antibodyAnti-Cd Antigen Cd45 antibodyAnti-Ptprc antibodyAnti-Ly-5 antibody |
Information sourced from Uniprot.org