| Host: |
Mouse |
| Applications: |
WB/ICC/IP |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-CD44 (Hyaluron Binding Region) is suitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
M588 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1 |
| Formulation: |
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: |
Protein G Purified |
| Dilution Range: |
WB 1:1000IHCICC 1:100IP 1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CD44 |
| Gene ID: |
960 |
| Uniprot ID: |
CD44_HUMAN |
| Immunogen Region: |
Hyaluron Binding Region |
| Specificity: |
Clone M588 detects 80-130 kDa* bands corresponding to the molecular mass of CD44 on SDS-PAGE immunoblots of native MDA-MB-231, A431, and A549 cell lysates. The antibody also detects denatured CD44 but with lower affinity. In addition, Clone M588 bind |
| Immunogen: |
Clone M588 was generated from a proprietary antigen related to the hyaluron binding region of human CD44 from the MDA-MB-231 breast cancer cell line. |
| Tissue Specificity | Detected in fibroblasts and urine (at protein level). Detected in placenta (at protein level). Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. |
| Post Translational Modifications | Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors. N-glycosylated. O-glycosylated.contains chondroitin sulfate glycans which can be more or less sulfated and whose number may affect the accessibility of specific proteinases to their cleavage site(s). It is uncertain if O-glycosylation occurs on Thr-637 or Thr-638. Phosphorylated.activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. |
| Function | Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment. Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection. Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases. Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion. |
| Protein Name | Cd44 AntigenCdw44EpicanExtracellular Matrix Receptor IiiEcmr-IiiGp90 Lymphocyte Homing/Adhesion ReceptorHutch-IHeparan Sulfate ProteoglycanHermes AntigenHyaluronate ReceptorPhagocytic Glycoprotein 1Pgp-1Phagocytic Glycoprotein IPgp-ICd Antigen Cd44 |
| Database Links | Reactome: R-HSA-1474228Reactome: R-HSA-202733Reactome: R-HSA-216083Reactome: R-HSA-2160916Reactome: R-HSA-6798695Reactome: R-HSA-877300 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCell ProjectionMicrovillusSecretedColocalizes With Actin In Membrane Protrusions At Wounding EdgesCo-Localizes With RdxEzr And Msn In MicrovilliLocalizes To Cholesterol-Rich Membrane-Bound Lipid Raft Domains |
| Alternative Antibody Names | Anti-Cd44 Antigen antibodyAnti-Cdw44 antibodyAnti-Epican antibodyAnti-Extracellular Matrix Receptor Iii antibodyAnti-Ecmr-Iii antibodyAnti-Gp90 Lymphocyte Homing/Adhesion Receptor antibodyAnti-Hutch-I antibodyAnti-Heparan Sulfate Proteoglycan antibodyAnti-Hermes Antigen antibodyAnti-Hyaluronate Receptor antibodyAnti-Phagocytic Glycoprotein 1 antibodyAnti-Pgp-1 antibodyAnti-Phagocytic Glycoprotein I antibodyAnti-Pgp-I antibodyAnti-Cd Antigen Cd44 antibodyAnti-CD44 antibodyAnti-LHR antibodyAnti-MDU2 antibodyAnti-MDU3 antibodyAnti-MIC4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
