Anti-Cd4 antibody [GK1.5] (STJA0001615)
SPECIFICATIONS
ClonalityMonoclonal
HostRat
ConjugationUnconjugated
IsotypeIgG2bk
General Information
| Short Description | Rat monoclonal anti-T-cell surface glycoprotein CD4 for use in Depletion, Block, FC, WB, IHC and IP in Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | Depletion/Block/FC/WB/IHC/IP |
| Host | Rat |
| Reactivity | Mouse |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | GK1.5 |
| Isotype | IgG2bk |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Formulation | 0.2 ยตm filtered in PBS, pH7.2. Azide Free (AF) /Low Endotoxin (LE) : Contains no stabilizers or preservatives. Endotoxin level is < 2 EU/mg as determined by LAL gel clotting assay. |
| Storage Instruction | This product can be stored at 2-8ยฐC for 12 months. Do not freeze. |
Target Information
| Gene Symbol | Cd4 |
| Gene ID | 12504 |
| Uniprot ID | CD4_MOUSE |
Additional Info
| Tissue Specificity | Highly expressed in T-helper cells. The presence of CD4 is a hallmark of T-helper cells which are specialized in the activation and growth of cytotoxic T-cells, regulation of B cells, or activation of phagocytes. CD4 is also present in other immune cells such as macrophages, dendritic cells or NK cells. |
| Post Translational Modifications | Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. Phosphorylated by PKC.phosphorylation plays an important role for CD4 internalization. |
| Function | Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages. |
| Protein Name | T-Cell Surface Glycoprotein Cd4T-Cell Differentiation Antigen L3t4T-Cell Surface Antigen T4/Leu-3Cd Antigen Cd4 |
| Database Links | Reactome: R-MMU-1462054Reactome: -MMU-202424Reactome: -MMU-202427Reactome: -MMU-202430Reactome: -MMU-202433Reactome: -MMU-389948Reactome: -MMU-449836Reactome: -MMU-8856825Reactome: -MMU-8856828 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-T-Cell Surface Glycoprotein Cd4 antibodyAnti-T-Cell Differentiation Antigen L3t4 antibodyAnti-T-Cell Surface Antigen T4/Leu-3 antibodyAnti-Cd Antigen Cd4 antibodyAnti-Cd4 antibody |
Information sourced from Uniprot.org