Anti-CD3G antibody (23-116) (STJ11103929)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal T-Cell Surface Glycoprotein Cd3 Gamma Chain (23-116) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CD3G |
| Gene ID | 917 |
| Uniprot ID | CD3G_HUMAN |
| Immunogen Region | 23-116 |
| Immunogen Sequence | QSIKGNHLVKVYDYQEDGSV LLTCDAEAKNITWFKDGKMI GFLTEDKKKWNLGSNAKDPR GMYQCKGSQNKSKPLQVYYR MCQNCIELNAATIS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 23-116 of human CD3G (NP_000064.1). |
Additional Info
| Post Translational Modifications | Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8. Phosphorylated also by PKC.leading to the TCR complex down-regulation. Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8. |
| Function | Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition to this role of signal transduction in T-cell activation, CD3G plays an essential role in the dynamic regulation of TCR expression at the cell surface. Indeed, constitutive TCR cycling is dependent on the di-leucine-based (diL) receptor-sorting motif present in CD3G. |
| Protein Name | T-Cell Surface Glycoprotein Cd3 Gamma ChainT-Cell Receptor T3 Gamma ChainCd Antigen Cd3g |
| Database Links | Reactome: R-HSA-198933Reactome: R-HSA-202424Reactome: R-HSA-202427Reactome: R-HSA-202430Reactome: R-HSA-202433Reactome: R-HSA-2029481Reactome: R-HSA-2029482Reactome: R-HSA-2029485Reactome: R-HSA-389948Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-9664323Reactome: R-HSA-9664422 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-T-Cell Surface Glycoprotein Cd3 Gamma Chain antibodyAnti-T-Cell Receptor T3 Gamma Chain antibodyAnti-Cd Antigen Cd3g antibodyAnti-CD3G antibodyAnti-T3G antibody |
Information sourced from Uniprot.org