| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Rat/Pig |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-CD3E-Cytoplasmic (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000. WB: Approx 22kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 23.1kDa according to NP_000724.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation w |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | CD3E |
| Gene ID: | 916 |
| Uniprot ID: | CD3E_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_000724.1 |
| Specificity: | This antibody is designed to recognize the cytoplasmic domain (aa153-207) of the protein. |
| Immunogen Sequence: | RKGQRDLYSGLNQR |
| Function | Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region. In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx. |
| Protein Name | T-Cell Surface Glycoprotein Cd3 Epsilon Chain T-Cell Surface Antigen T3/Leu-4 Epsilon Chain Cd Antigen Cd3e |
| Database Links | Reactome: R-HSA-198933 Reactome: R-HSA-202424 Reactome: R-HSA-202427 Reactome: R-HSA-202430 Reactome: R-HSA-202433 Reactome: R-HSA-389948 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-T-Cell Surface Glycoprotein Cd3 Epsilon Chain antibody Anti-T-Cell Surface Antigen T3/Leu-4 Epsilon Chain antibody Anti-Cd Antigen Cd3e antibody Anti-CD3E antibody Anti-T3E antibody |
Information sourced from Uniprot.org