• Immunofluorescence analysis of Jurkat cells using CD3D antibody (STJ22995) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat spleen using CD3D antibody (STJ22995) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Jurkat cells, using CD3D antibody (STJ22995) at 1:500 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.

Anti-CD3D antibody (22-105) (STJ22995)

SKU:
STJ22995

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD3D (22-105) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD3D
Gene ID: 915
Uniprot ID: CD3D_HUMAN
Immunogen Region: 22-105
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 22-105 of human CD3D (NP_000723.1).
Immunogen Sequence: FKIPIEELEDRVFVNCNTSI TWVEGTVGTLLSDITRLDLG KRILDPRGIYRCNGTDIYKD KESTVQVHYRMCQSCVELDP ATVA
Tissue Specificity CD3D is mostly present on T-lymphocytes with its TCR-CD3 partners. Present also in fetal NK-cells.
Post Translational Modifications Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.
Function Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells.
Protein Name T-Cell Surface Glycoprotein Cd3 Delta Chain
T-Cell Receptor T3 Delta Chain
Cd Antigen Cd3d
Database Links Reactome: R-HSA-198933
Reactome: R-HSA-202424
Reactome: R-HSA-202427
Reactome: R-HSA-202430
Reactome: R-HSA-202433
Reactome: R-HSA-389948
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-T-Cell Surface Glycoprotein Cd3 Delta Chain antibody
Anti-T-Cell Receptor T3 Delta Chain antibody
Anti-Cd Antigen Cd3d antibody
Anti-CD3D antibody
Anti-T3D antibody

Information sourced from Uniprot.org

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