• Anti-CD31/PECAM1 antibody [158-2B3] {PE} (STJA0002655)

Anti-CD31/PECAM1 antibody [158-2B3] {PE} (STJA0002655)

SKU:
STJA0002655

£135.50 - £306.50
Current Stock:
Host: Mouse
Applications: FC
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Platelet endothelial cell adhesion molecule is suitable for use in Flow Cytometry research applications.
Clonality: Monoclonal
Clone ID: 158-2B3
Conjugation: PE
Isotype: IgG1k
Formulation: PBS with 0.05% Proclin300, 1% BSA
Storage Instruction: Recommend storing between 2-8°C and protecting from prolonged exposure to light. Do not freeze this product.
Gene Symbol: PECAM1
Gene ID: 5175
Uniprot ID: PECA1_HUMAN
Tissue Specificity Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Expressed in human umbilical vein endothelial cells (HUVECs) (at protein level). Expressed on neutrophils (at protein level). Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U-937 histiocytic lymphoma cell lines (at protein level).
Post Translational Modifications Phosphorylated on Ser and Tyr residues after cellular activation by src kinases. Upon activation, phosphorylated on Ser-729 which probably initiates the dissociation of the membrane-interaction segment (residues 709-729) from the cell membrane allowing the sequential phosphorylation of Tyr-713 and Tyr-690. Constitutively phosphorylated on Ser-734 in resting platelets. Phosphorylated on tyrosine residues by FER and FES in response to FCER1 activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation. Palmitoylation by ZDHHC21 is necessary for cell surface expression in endothelial cells and enrichment in membrane rafts.
Function Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions. Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils. Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal. Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells.PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes. Modulates bradykinin receptor BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells. Induces susceptibility to atherosclerosis. Isoform Delta15: Does not protect against apoptosis.
Protein Name Platelet Endothelial Cell Adhesion Molecule
Pecam-1
Endocam
Gpiia'
Peca1
Cd Antigen Cd31
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-202733
Reactome: R-HSA-210990
Reactome: R-HSA-216083
Reactome: R-HSA-432142
Reactome: R-HSA-6798695
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Cell Surface Expression On Neutrophils Is Down-Regulated Upon Fmlp Or Cxcl8/Il8-Mediated Stimulation
Isoform Long: Cell Membrane
Membrane Raft
Cell Junction
Localizes To The Lateral Border Recycling Compartment (Lbrc) And Recycles From The Lbrc To The Junction In Resting Endothelial Cells
Isoform Delta15: Cell Junction
Alternative Antibody Names Anti-Platelet Endothelial Cell Adhesion Molecule antibody
Anti-Pecam-1 antibody
Anti-Endocam antibody
Anti-Gpiia' antibody
Anti-Peca1 antibody
Anti-Cd Antigen Cd31 antibody
Anti-PECAM1 antibody

Information sourced from Uniprot.org

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