• STJ72503 (0. 3µg/ml) staining of Human Tonsil lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • EB010740 (2µg/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with citrate buffer Ph 6, HRP-staining.
  • STJ72503 Negative Control showing staining of paraffin embedded Human Lymph Node, with no primary antibody.

Anti-CD28 antibody (Internal) (STJ72503)

SKU:
STJ72503-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-CD28 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.3-1µg/ml
IHC-1-2µg/ml
ELISA-antibody detection limit dilution 1:64000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: CD28
Gene ID: 940
Uniprot ID: CD28_HUMAN
Immunogen Region: Internal
Accession Number: NP_006130.1
Specificity: The immunizing peptide corresponds to a part of the extracellular domain.
Immunogen Sequence: SQQLQVYSKTGFNCD
Post Translational Modifications CD40LG induces tyrosine phosphorylation of isoform 3.
Function Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation. Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells.
Protein Name T-Cell-Specific Surface Glycoprotein Cd28
Tp44
Cd Antigen Cd28
Database Links Reactome: R-HSA-1257604
Reactome: R-HSA-164939
Reactome: R-HSA-2219530
Reactome: R-HSA-389356
Reactome: R-HSA-389357
Reactome: R-HSA-389359
Reactome: R-HSA-6811558
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Isoform 3: Cell Surface
Alternative Antibody Names Anti-T-Cell-Specific Surface Glycoprotein Cd28 antibody
Anti-Tp44 antibody
Anti-Cd Antigen Cd28 antibody
Anti-CD28 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance