| Host: |
Mouse |
| Applications: |
FC/FUNC/IF/IHC |
| Reactivity: |
Cow/Human/Mouse/Pig/Sheep |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-CD28 is suitable for use in Flow Cytometry, FUNC, Immunofluorescence and Immunohistochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
204-12 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG2bk |
| Formulation: |
PBS with 0.02% Sodium Azide. |
| Purification: |
Affinity purified from tissue culture. |
| Concentration: |
Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Dilution Range: |
Flow cytometry (0, 5-1, 0 µg/million cells in 0, 1 ml). Functional studies (0, 02-2, 0 µg/ml without azide). Immunofluorescence (0, 5-1, 0 µg/ml). Immunohistology (1-2 µg/ml for 30-60 min at RT; for staining of formalin-fixed tissues no suitable anti |
| Storage Instruction: |
Store for up to 1 year at 2-8°C upon receipt. |
| Gene Symbol: |
CD28 |
| Gene ID: |
940 |
| Uniprot ID: |
CD28_HUMAN |
| Immunogen: |
A BALB/c mouse was immunized with human leucocytes. Fusion partner: NS-1. |
| Post Translational Modifications | Phosphorylated by LCK. Dephosphorylated by PTPN11. Isoform 3: Tyrosine phosphorylated induced by CD40LG. |
| Function | Receptor that plays a role in T-cell activation, proliferation, survival and the maintenance of immune homeostasis. Functions not only as an amplifier of TCR signals but delivers unique signals that control intracellular biochemical events that alter the gene expression program of T-cells. Stimulation upon engagement of its cognate ligands CD80 or CD86 increases proliferation and expression of various cytokines in particular IL2 production in both CD4(+) and CD8(+) T-cell subsets. Mechanistically, ligation induces recruitment of protein kinase C-theta/PRKCQ and GRB2 leading to NF-kappa-B activation via both PI3K/Akt-dependent and -independent pathways. In conjunction with TCR/CD3 ligation and CD40L costimulation, enhances the production of IL4 and IL10 in T-cells. Isoform 3: Enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells. |
| Protein Name | T-Cell-Specific Surface Glycoprotein Cd28Tp44Cd Antigen Cd28 |
| Database Links | Reactome: R-HSA-1257604Reactome: R-HSA-164939Reactome: R-HSA-2219530Reactome: R-HSA-389356Reactome: R-HSA-389357Reactome: R-HSA-389359Reactome: R-HSA-6811558 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinIsoform 3: Cell Surface |
| Alternative Antibody Names | Anti-T-Cell-Specific Surface Glycoprotein Cd28 antibodyAnti-Tp44 antibodyAnti-Cd Antigen Cd28 antibodyAnti-CD28 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
