| Host: | Mouse |
| Applications: | WB-Nat/ICC/IP |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-CD244/2B4/SLAMF4 M085 (Extracellular region) is suitable for use in Western Blot-Nat, Immunocytochemistry and Immunoprecipitation research applications. |
| Clonality : | Monoclonal |
| Clone ID : | M085 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Formulation: | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: | Protein G Purified |
| Dilution Range: | WB 1:1000 ICC 1:200 IP 1:100 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CD244 |
| Gene ID: | 51744 |
| Uniprot ID: | CD244_HUMAN |
| Immunogen Region: | Extracellular region |
| Specificity: | Clone M085 mouse monoclonal antibody detects a 110 kDa* protein on SDS-PAGE Native" immunoblots of human THP1 monocytes. The antibody detects only the native CD244 protein, and the aldehyde-fixed CD244 protein. The antibody works for native western b |
| Immunogen: | Clone (M085) was generated from a recombinant protein that included the extracellular region of human CD244 protein. |
| Tissue Specificity | Expressed in spleen, PBL, followed by lung, liver, testis and small intestine. Expressed in all natural killer (NK) cells, monocytes and basophils, TCR-gamma/delta+ T-cells, monocytes, basophils, and on a subset of CD8(+) T-cells. |
| Post Translational Modifications | N-linked glycosylation is essential for the binding to its ligand CD48. Also O-glycosylated, in contrast, O-linked sialylation has a negative impact on ligand binding. Phosphorylated by FYN and CSK on tyrosine residues following activation. Coligation with inhibitory receptors such as KIR2DL1 inhibits phosphorylation upon contact of NK cells with sensitive target cells. |
| Function | Heterophilic receptor of the signaling lymphocytic activation molecule (SLAM) family.its ligand is CD48. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Acts as activating natural killer (NK) cell receptor. Activating function implicates association with SH2D1A and FYN. Downstreaming signaling involves predominantly VAV1, and, to a lesser degree, INPP5D/SHIP1 and CBL. Signal attenuation in the absence of SH2D1A is proposed to be dependent on INPP5D and to a lesser extent PTPN6/SHP-1 and PTPN11/SHP-2. Stimulates NK cell cytotoxicity, production of IFN-gamma and granule exocytosis. Optimal expansion and activation of NK cells seems to be dependent on the engagement of CD244 with CD48 expressed on neighboring NK cells. Acts as costimulator in NK activation by enhancing signals by other NK receptors such as NCR3 and NCR1. At early stages of NK cell differentiation may function as an inhibitory receptor possibly ensuring the self-tolerance of developing NK cells. Involved in the regulation of CD8(+) T-cell proliferation.expression on activated T-cells and binding to CD48 provides costimulatory-like function for neighboring T-cells. Inhibits inflammatory responses in dendritic cells (DCs). |
| Protein Name | Natural Killer Cell Receptor 2b4 Nk Cell Activation-Inducing Ligand Nail Nk Cell Type I Receptor Protein 2b4 Nkr2b4 H2b4 Slam Family Member 4 Slamf4 Signaling Lymphocytic Activation Molecule 4 Cd Antigen Cd244 |
| Database Links | Reactome: R-HSA-202733 |
| Cellular Localisation | Membrane Single-Pass Type I Membrane Protein Cell Membrane Membrane Raft Receptor Engagement Results In A Recruitment To Lipid Drafts Essential For The Subsequent Tyrosine Phosphorylation Of The Itsms |
| Alternative Antibody Names | Anti-Natural Killer Cell Receptor 2b4 antibody Anti-Nk Cell Activation-Inducing Ligand antibody Anti-Nail antibody Anti-Nk Cell Type I Receptor Protein 2b4 antibody Anti-Nkr2b4 antibody Anti-H2b4 antibody Anti-Slam Family Member 4 antibody Anti-Slamf4 antibody Anti-Signaling Lymphocytic Activation Molecule 4 antibody Anti-Cd Antigen Cd244 antibody Anti-CD244 antibody Anti-2B4 antibody |
Information sourced from Uniprot.org