• Immunohistochemistry analysis of paraffin-embedded human oophoroma using CD239/BCAM Rabbit monoclonal antibody (STJ11103094) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using CD239/BCAM Rabbit monoclonal antibody (STJ11103094) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CD239/BCAM antibody (529-628) [S4MR] (STJ11103094)

SKU:
STJ11103094

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CD239/BCAM (529-628) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BCAM
Gene ID: 4059
Uniprot ID: BCAM_HUMAN
Immunogen Region: 529-628
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 529-628 of human CD239/BCAM (P50895).
Immunogen Sequence: GNKRHVFHFGTVSPQTSQAG VAVMAVAVSVGLLLLVVAVF YCVRRKGGPCCRQRREKGAP PPGEPGLSHSGSEQPEQTGL LMGGASGGARGGSGGFGDEC
Tissue Specificity Wide tissue distribution (highest in the pancreas and very low in brain). Closely associated with the basal layer of cells in epithelia and the endothelium of blood vessel walls.
Post Translational Modifications Epinephrine-stimulated phosphorylation of Ser-621 by PKA enhances adhesion to laminin.
Function Laminin alpha-5 receptor. May mediate intracellular signaling.
Protein Name Basal Cell Adhesion Molecule
Auberger B Antigen
B-Cam Cell Surface Glycoprotein
F8/G253 Antigen
Lutheran Antigen
Lutheran Blood Group Glycoprotein
Cd Antigen Cd239
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Basal Cell Adhesion Molecule antibody
Anti-Auberger B Antigen antibody
Anti-B-Cam Cell Surface Glycoprotein antibody
Anti-F8/G253 Antigen antibody
Anti-Lutheran Antigen antibody
Anti-Lutheran Blood Group Glycoprotein antibody
Anti-Cd Antigen Cd239 antibody
Anti-BCAM antibody
Anti-LU antibody
Anti-MSK19 antibody

Information sourced from Uniprot.org

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