Anti-CD221 IGF Receptor antibody [1H7] (STJ16101843)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG
ImmunogenPurified human placental CD221 IGF-1 receptor
General Information
| Short Description | Mouse monoclonal anti-CD221 IGF Receptor for use in IHC-P, FC and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-P/FC/WB |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 1H7 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Purified |
| Dilution Range | IHC-P 1:10-1:40FC 1:10WB |
| Formulation | PBS with azide |
| Storage Instruction | Store at 2-8°C for up to 1-year, upon receipt. |
Target Information
| Immunogen | Purified human placental CD221 IGF-1 receptor |
Additional Info
| Background | IGF-1 Receptor recognizes human CD221, a 155kD receptor tyrosine kinase, also known as Insulin-like growth factor I receptor (IGF-I Receptor). CD221 is composed of two extracellular alpha-subunits and two transmembrane beta-subunits. Clone 1H7 recognizes an epitope in the alpha subunits of CD221, demonstrated by Western blotting (1). CD221 is expressed in a range of tissues, including kidney, liver, placenta, mammary gland, brain, ovary and skin. The ligands for CD221 include IGF-I and IGF-II, which bind to CD221 and activate tyrosine kinase activity, resulting in phosphorylation of several intracellular signaling proteins. Clone 1H7 is reported to partically block binding of IGF-I and IGF-II to CD221 (1). Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Pretreatment: protein digestion (Trypsin or Pronase) is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. |
Information sourced from Uniprot.org