• STJ70052 (0. 05µg/ml) staining of Human Lymph Node lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70052 (0. 01µg/ml) staining of Daudi (A) and negative control HepG2 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70052 Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells, immobilized on Shi-fix™ coverslip. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70052 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70052 Flow cytometric analysis of paraformaldehyde fixed Daudi cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
  • STJ70052 (2µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. This data is from a previous batch, not on sale.

Anti-CD20/MS4A1 antibody (C-Term) (STJ70052)

SKU:
STJ70052-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-CD20/MS4A1 (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.01-0.05µg/ml
IF-Strong expression of the protein seen in the membranes of Jurkat and in the nuclei and cytoplasm of U2OS cells. 10µg/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: MS4A1
Gene ID: 931
Uniprot ID: CD20_HUMAN
Immunogen Region: C-Term
Accession Number: NP_068769.2; NP_690605.1
Specificity: Please note that both reported variants (NP_068769.2 and NP_690605.1) encode the same protein.
Immunogen Sequence: CQDQESSPIENDSSP
Post Translational Modifications Phosphorylated on serines and threonines in resting B-cells. Protein kinase C/PKC can use CD20 as substrate.
Function B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR.
Protein Name B-Lymphocyte Antigen Cd20
B-Lymphocyte Surface Antigen B1
Bp35
Leukocyte Surface Antigen Leu-16
Membrane-Spanning 4-Domains Subfamily A Member 1
Cd Antigen Cd20
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Lipid-Anchor
Constitutively Associated With Membrane Rafts
Alternative Antibody Names Anti-B-Lymphocyte Antigen Cd20 antibody
Anti-B-Lymphocyte Surface Antigen B1 antibody
Anti-Bp35 antibody
Anti-Leukocyte Surface Antigen Leu-16 antibody
Anti-Membrane-Spanning 4-Domains Subfamily A Member 1 antibody
Anti-Cd Antigen Cd20 antibody
Anti-MS4A1 antibody
Anti-CD20 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance