| Short Description : | Mouse monoclonal anti-CD1b for use in ELISA, FC, IF and IHC in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | ELISA/FC/IF/IHC |
| Host: | Mouse |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | 100-1A5 |
| Isotype: | IgMk |
| Conjugation: | Unconjugated |
| Concentration: | Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Purification: | Affinity purified from tissue culture. |
| Dilution Range: | ELISA (solid phase: not known; tracer: 0.001-100 µg/ml for 30 min at RT). Flow cytometry (0.5-1.0 µg/million cells in 0.1 ml). Immunofluorescence (0.5-1.0 µg/ml). Immunohistology (1.0-2.0 µg/ml for 30 min at RT; an appropriate antigen retrieval metho |
| Formulation: | PBS with 0.02% Sodium Azide. |
| Storage Instruction: | Store for up to 1 year at 2-8°C upon receipt. |
| Immunogen: | A BALB/c mouse was immunized with stimulated human leucocytes. Fusion partner: NS-1. |
| Background | 100-1A5 reacts with CD1b, a 44KDa type 1 glycoprotein associated with beta2-microglobulin. It is expressed on dendritic cells, Langerhans cells, thymocytes and T acute lymphoblastic leukemia cells. CD1, type 1 membrane protein, has structural similarity to the MHC class 1 molecule and has been shown to present lipid antigens for recognition by T lymphocytes. CD1b is also expressed in Langerhans interdigitating cells. 100-1A5 also reacts with pyramidal cells in the brain and was typed at the IVth International CD Workshop. |
Information sourced from Uniprot.org
