Anti-CD19 antibody [EBS-CD-015] (STJ16102585)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1k
ImmunogenA BALB/c mouse was immunized with NALM1 + NALM16 cells. Fusion partner: Sp2/0.
General Information
| Short Description | Mouse monoclonal anti-CD19 for use in FC, IF and IHC in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC/IF/IHC |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | EBS-CD-015 |
| Isotype | IgG1k |
| Conjugation | Unconjugated |
| Concentration | Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Purification | Affinity purified from tissue culture. |
| Dilution Range | Flow Cytometry (0, 5-2, 0 Mu g/million cells in 0, 1ml for 30 min, at 4°C). Immunofluorescence (0, 5-2, 0 Mu g/ml). Immunohistology (1-2 Mu g/ml for 30 min at RT; no suitable antigen retrieval procedure is known to date for formalin-fixed tissues). |
| Formulation | PBS with 0.02% Sodium Azide. |
| Storage Instruction | Store for up to 1 year at 2-8°C upon receipt. |
Target Information
| Gene Symbol | Cd19 |
| Gene ID | 12478 |
| Uniprot ID | CD19_MOUSE |
| Immunogen | A BALB/c mouse was immunized with NALM1 + NALM16 cells. Fusion partner: Sp2/0. |
Additional Info
| Post Translational Modifications | Phosphorylated on tyrosine following B-cell activation. Phosphorylated on tyrosine residues by LYN. Tyrosine residues are phosphorylated sequentially after activation of the B cell receptor. Phosphorylation of Tyr-522 is extremely rapid, followed by phosphorylation at Tyr-402. In contrast, phosphorylation of Tyr-493 appears more slowly and is more transient, returning rapidly to basal levels. |
| Function | Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores. Is not required for early steps during B cell differentiation in the blood marrow. Required for normal differentiation of B-1 cells. Required for normal B cell differentiation and proliferation in response to antigen challenges. Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge. |
| Protein Name | B-Lymphocyte Antigen Cd19Differentiation Antigen Cd19Cd Antigen Cd19 |
| Database Links | Reactome: R-MMU-1257604Reactome: -MMU-198933Reactome: -MMU-6811558Reactome: -MMU-977606Reactome: -MMU-983695 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinMembrane Raft |
| Alternative Antibody Names | Anti-B-Lymphocyte Antigen Cd19 antibodyAnti-Differentiation Antigen Cd19 antibodyAnti-Cd Antigen Cd19 antibodyAnti-Cd19 antibody |
Information sourced from Uniprot.org