| Host: | Mouse |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-B-lymphocyte antigen CD19 is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Monoclonal |
| Clone ID : | 2E2B6B10 |
| Conjugation: | Unconjugated |
| Isotype: | IgG2a |
| Formulation: | Liquid in PBS containing 0.03% Sodium Azide. |
| Purification: | Affinity purification |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:200-1:1000 ELISA 1:10000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CD19 |
| Gene ID: | 930 |
| Uniprot ID: | CD19_HUMAN |
| Specificity: | CD19 Monoclonal Antibody detects endogenous levels of CD19 protein. |
| Immunogen: | Purified recombinant fragment of human CD19 expressed in E. Coli. |
| Post Translational Modifications | Phosphorylated on tyrosine following B-cell activation. Phosphorylated on tyrosine residues by LYN. Tyrosine residues are phosphorylated sequentially after activation of the B cell receptor. Phosphorylation of Tyr-531 is extremely rapid, followed by phosphorylation at Tyr-409. In contrast, phosphorylation of Tyr-500 appears more slowly and is more transient, returning rapidly to basal levels. |
| Function | Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores. Is not required for early steps during B cell differentiation in the blood marrow. Required for normal differentiation of B-1 cells. Required for normal B cell differentiation and proliferation in response to antigen challenges. Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge. |
| Protein Name | B-Lymphocyte Antigen Cd19 B-Lymphocyte Surface Antigen B4 Differentiation Antigen Cd19 T-Cell Surface Antigen Leu-12 Cd Antigen Cd19 |
| Database Links | Reactome: R-HSA-1257604 Reactome: R-HSA-198933 Reactome: R-HSA-2219530 Reactome: R-HSA-6811558 Reactome: R-HSA-977606 Reactome: R-HSA-983695 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Membrane Raft |
| Alternative Antibody Names | Anti-B-Lymphocyte Antigen Cd19 antibody Anti-B-Lymphocyte Surface Antigen B4 antibody Anti-Differentiation Antigen Cd19 antibody Anti-T-Cell Surface Antigen Leu-12 antibody Anti-Cd Antigen Cd19 antibody Anti-CD19 antibody |
Information sourced from Uniprot.org