Host: | Mouse |
Applications: | IHC-P |
Reactivity: | Human |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Mouse monoclonal antibody anti-CD14 (25-148aa) is suitable for use in Immunohistochemistry research applications. |
Clonality: | Monoclonal |
Clone ID: | ZM104 |
Conjugation: | Unconjugated |
Isotype: | IgG1/Kappa |
Formulation: | Tris-HCI buffer containing stabilizing protein (BSA) and <0.1% ProClin |
Purification: | Affinity purified |
Dilution Range: | 1:100-200 |
Storage Instruction: | Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C. |
Gene Symbol: | CD14 |
Gene ID: | 929 |
Uniprot ID: | CD14_HUMAN |
Immunogen Region: | 25-148aa |
Specificity: | Positive control: Tonsil or lymph node |
Immunogen: | Recombinant fragment of human CD14 protein (around aa 25-148) |
Tissue Specificity | Detected on macrophages (at protein level). Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes.also expressed by most tissue macrophages. |
Post Translational Modifications | N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. |
Function | Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-). |
Protein Name | Monocyte Differentiation Antigen Cd14 My23 Antigen Myeloid Cell-Specific Leucine-Rich Glycoprotein Cd Antigen Cd14 Cleaved Into - Monocyte Differentiation Antigen Cd14 - Urinary Form - Monocyte Differentiation Antigen Cd14 - Membrane-Bound Form |
Database Links | Reactome: R-HSA-1236974 Reactome: R-HSA-140534 Reactome: R-HSA-166016 Reactome: R-HSA-166020 Reactome: R-HSA-166058 Reactome: R-HSA-166166 Reactome: R-HSA-168179 Reactome: R-HSA-168188 Reactome: R-HSA-2562578 Reactome: R-HSA-5602498 Reactome: R-HSA-5603041 Reactome: R-HSA-5686938 Reactome: R-HSA-6798695 Reactome: R-HSA-936964 Reactome: R-HSA-937041 Reactome: R-HSA-937072 Reactome: R-HSA-975163 Reactome: R-HSA-9820960 Reactome: R-HSA-9824878 Reactome: R-HSA-9833110 |
Cellular Localisation | Cell Membrane Lipid-Anchor Gpi-Anchor Secreted Membrane Raft Golgi Apparatus Secreted Forms May Arise By Cleavage Of The Gpi Anchor |
Alternative Antibody Names | Anti-Monocyte Differentiation Antigen Cd14 antibody Anti-My23 Antigen antibody Anti-Myeloid Cell-Specific Leucine-Rich Glycoprotein antibody Anti-Cd Antigen Cd14 Cleaved Into - Monocyte Differentiation Antigen Cd14 - Urinary Form - Monocyte Differentiation Antigen Cd14 - Membrane-Bound Form antibody Anti-CD14 antibody |
Information sourced from Uniprot.org