Western blot analysis of various lysates, using CD109 Rabbit monoclonal antibody (STJ11105222) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC) :K-562Exposure time: 60s.

Anti-CD109 antibody (223-462 aa) [S5222RM] (STJ11105222)

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STJ11105222
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Monoclonal
Clone ID : S5222RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD109
Gene ID: 135228
Uniprot ID: CD109_HUMAN
Immunogen Region: 223-462 aa
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 223-462 of human CD109. (NP_598000.2).
Immunogen Sequence: EYVLPKFEVTLQTPLYCSMN SKHLNGTITAKYTYGKPVKG DVTLTFLPLSFWGKKKNITK TFKINGSANFSFNDEEMKNV MDSSNGLSEYLDLSSPGPVE ILTTVTESVTGISRNVSTNV FFKQHDYIIEFFDYTTVLKP SLNFTATVKVTRADGNQLTL EERRNNVVITVTQRNYTEYW SGSNSGNQKMEAVQKINYTV PQSGTFKIEFPILEDSSELQ LKAYFLGSKSSMAVHSLFK
Tissue Specificity Widely expressed with high level in uterus, aorta, heart, lung, trachea, placenta and in fetal heart, kidney, liver, spleen and lung. Expressed by CD34(+) acute myeloid leukemia cell lines, T-cell lines, activated T-lymphoblasts, endothelial cells and activated platelets. Isoform 4 is expressed in placenta. Isoform 1 is expressed in keratinocytes and placenta.
Post Translational Modifications N-glycosylated. 2 forms of 150 (p150) and 120 kDa (p120) exist due to proteolytic degradation from a 180 kDa form.
Function Modulates negatively TGFB1 signaling in keratinocytes.
Protein Name Cd109 Antigen
150 Kda Tgf-Beta-1-Binding Protein
C3 And Pzp-Like Alpha-2-Macroglobulin Domain-Containing Protein 7
Platelet-Specific Gov Antigen
P180
R150
Cd Antigen Cd109
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-163125
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Alternative Antibody Names Anti-Cd109 Antigen antibody
Anti-150 Kda Tgf-Beta-1-Binding Protein antibody
Anti-C3 And Pzp-Like Alpha-2-Macroglobulin Domain-Containing Protein 7 antibody
Anti-Platelet-Specific Gov Antigen antibody
Anti-P180 antibody
Anti-R150 antibody
Anti-Cd Antigen Cd109 antibody
Anti-CD109 antibody
Anti-CPAMD7 antibody

Information sourced from Uniprot.org

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