Anti-CCNT1 antibody (220-320) (STJ22961)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CCNT1 (220-320) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:5000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CCNT1 |
| Gene ID | 904 |
| Uniprot ID | CCNT1_HUMAN |
| Immunogen Region | 220-320 |
| Immunogen Sequence | HWWEYVDATVTLELLDELTH EFLQILEKTPNRLKRIWNWR ACEAAKKTKADDRGTDEKTS EQTILNMISQSSSDTTIAGL MSMSTSTTSAVPSLPVSEES S |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 220-320 of human Cyclin T1 (NP_001231.2). |
Additional Info
| Tissue Specificity | Ubiquitously expressed. |
| Post Translational Modifications | ADP-ribosylation on serine residues by PARP1 in response to DNA damage disrupts the phase separation activity of CCNT1, thereby preventing activation of CDK9. |
| Function | Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). Required to activate the protein kinase activity of CDK9: acts by mediating formation of liquid-liquid phase separation (LLPS) that enhances binding of P-TEFb to the CTD of RNA Pol II. (Microbial infection) In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes. |
| Protein Name | Cyclin-T1Cyct1Cyclin-T |
| Database Links | Reactome: R-HSA-112382Reactome: R-HSA-167152Reactome: R-HSA-167200Reactome: R-HSA-167238Reactome: R-HSA-167243Reactome: R-HSA-167246Reactome: R-HSA-167287Reactome: R-HSA-167290Reactome: R-HSA-176034Reactome: R-HSA-2173796Reactome: R-HSA-674695Reactome: R-HSA-6796648Reactome: R-HSA-6807505Reactome: R-HSA-75955Reactome: R-HSA-9018519 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Cyclin-T1 antibodyAnti-Cyct1 antibodyAnti-Cyclin-T antibodyAnti-CCNT1 antibody |
Information sourced from Uniprot.org