• Immunohistochemistry analysis of Cyclin E2 in paraffin-embedded mouse testis using Cyclin E2 Rabbit polyclonal antibody (STJ22953) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Cyclin E2 in paraffin-embedded mouse lung using Cyclin E2 Rabbit polyclonal antibody (STJ22953) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Cyclin E2 in paraffin-embedded rat testis using Cyclin E2 Rabbit polyclonal antibody (STJ22953) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Cyclin E2 Rabbit polyclonal antibody (STJ22953) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-CCNE2 antibody (255-404) (STJ22953)

SKU:
STJ22953

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CCNE2 (255-404) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CCNE2
Gene ID: 9134
Uniprot ID: CCNE2_HUMAN
Immunogen Region: 255-404
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 255-404 of human Cyclin E2 (NP_477097.1).
Immunogen Sequence: ALKDAPKVLLPQYSQETFIQ IAQLLDLCILAIDSLEFQYR ILTAAALCHFTSIEVVKKAS GLEWDSISECVDWMVPFVNV VKSTSPVKLKTFKKIPMEDR HNIQTHTNYLAMLEEVNYIN TFRKGGQLSPVCNGGIMTPP KSTEKPPGKH
Tissue Specificity highest levels of expression in adult testis, thymus and brain. Lower levels in placenta, spleen and colon. Consistently elevated levels in tumor-derived cells compared to non-transformed proliferating cells. : low levels in thymus, prostate, brain, skeletal muscle, and kidney. Elevated levels in lung. highly expressed in testis, placenta, thymus and brain. In a lesser extent in small intestine and colon.
Post Translational Modifications Phosphorylation by CDK2 triggers its release from CDK2 and degradation via the ubiquitin proteasome pathway.
Function Essential for the control of the cell cycle at the late G1 and early S phase.
Protein Name G1/S-Specific Cyclin-E2
Database Links Reactome: R-HSA-1538133
Reactome: R-HSA-187577
Reactome: R-HSA-2559586
Reactome: R-HSA-390471
Reactome: R-HSA-6804116
Reactome: R-HSA-69017
Reactome: R-HSA-69200
Reactome: R-HSA-69202
Reactome: R-HSA-69231
Reactome: R-HSA-69563
Reactome: R-HSA-9661069
Cellular Localisation Nucleus
Alternative Antibody Names Anti-G1/S-Specific Cyclin-E2 antibody
Anti-CCNE2 antibody

Information sourced from Uniprot.org

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