Host: |
Rabbit |
Applications: |
WB |
Reactivity: |
Human/Mouse |
Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: |
Rabbit polyclonal antibody anti-Cyclin D3 (201-292) is suitable for use in Western Blot research applications. |
Clonality: |
Polyclonal |
Conjugation: |
Unconjugated |
Isotype: |
IgG |
Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
Purification: |
Affinity purification |
Dilution Range: |
WB 1:500-1:2000 |
Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: |
CCND3 |
Gene ID: |
896 |
Uniprot ID: |
CCND3_HUMAN |
Immunogen Region: |
201-292 |
Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 201-292 of human Cyclin D3 (NP_001751.1). |
Immunogen Sequence: |
SMIATGSIGAAVQGLGACSM SGDELTELLAGITGTEVDCL RACQEQIEAALRESLREASQ TSSSPAPKAPRGSSSQGPSQ TSTPTDVTAIHL |
Post Translational Modifications | Phosphorylation at Thr-283 by MAP kinases is required for ubiquitination and degradation by the DCX(AMBRA1) complex. Ubiquitinated by the DCX(AMBRA1) complex during the transition from G1 to S cell phase, leading to its degradation: ubiquitination is dependent on Thr-283 phosphorylation. The DCX(AMBRA1) complex represents the major regulator of CCND3 stability during the G1/S transition. Polyubiquitinated by the SCF(FBXL2) complex, leading to proteasomal degradation. |
Function | Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Shows transcriptional coactivator activity with ATF5 independently of CDK4. |
Protein Name | G1/S-Specific Cyclin-D3 |
Database Links | Reactome: R-HSA-381340Reactome: R-HSA-5687128Reactome: R-HSA-69231Reactome: R-HSA-8934593Reactome: R-HSA-9661069Reactome: R-HSA-9754119 |
Cellular Localisation | NucleusCytoplasm |
Alternative Antibody Names | Anti-G1/S-Specific Cyclin-D3 antibodyAnti-CCND3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance