Western blot analysis of lysates from RAW 264. 7 cells using CCL4 Rabbit pAb (STJ11107114) at 1:1000 dilutionย incubated overnight at 4C. Raw264. 7 cells were treated by PMA (80 nM) at 37C for overnight and LPS (1 Mu g/ml) at 37C for 6 hours and Brefeldin A for 3 hours of stimulation. <br> Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 30 Mu g per lane. <br> Blocking buffer: 3 % nonfat dry milk in TBST. <br> Detection: ECL Basic Kit <br> Exposure time: 30s.

Anti-CCL4 antibody (24-92 aa) (STJ11107114)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11107114
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General Information

Short DescriptionRabbit polyclonal CCL4 (24-92 aa) antibody for use in WB and ELISA in mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityMouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolCcl4
Gene ID20303
Uniprot IDCCL4_MOUSE
Immunogen Region24-92 aa
Immunogen SequenceAPMGSDPPTSCCFSYTSRQL HRSFVMDYYETSSLCSKPAV VFLTKRGRQICANPSEPWVT EYMSDLELN
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 24-92 of mouse CCL4 (NP_038680.1).

Additional Info

Function Monokine with inflammatory and chemokinetic properties.
Protein Name C-C Motif Chemokine 4
Immune Activation Protein 2
Act-2
Act2
Macrophage Inflammatory Protein 1-Beta
Mip-1-Beta
Protein H400
Sis-Gamma
Small-Inducible Cytokine A4
Database Links Reactome: R-MMU-380108
Reactome: -MMU-418594
Cellular Localisation Secreted
Alternative Antibody Names Anti-C-C Motif Chemokine 4 antibody
Anti-Immune Activation Protein 2 antibody
Anti-Act-2 antibody
Anti-Act2 antibody
Anti-Macrophage Inflammatory Protein 1-Beta antibody
Anti-Mip-1-Beta antibody
Anti-Protein H400 antibody
Anti-Sis-Gamma antibody
Anti-Small-Inducible Cytokine A4 antibody
Anti-Ccl4 antibody
Anti-Mip1b antibody
Anti-Scya4 antibody

Information sourced from Uniprot.org

Citations

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