• Immunohistochemistry analysis of CCL25 in paraffin-embedded Rat large intestine tissue using CCL25 Rabbit polyclonal antibody (STJ11105643) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of CCL25 in paraffin-embedded Mouse large intestine tissue using CCL25 Rabbit polyclonal antibody (STJ11105643) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of lysates from wild type (WT) and 293T cells transfected with CCL25 using CCL25 Rabbit polyclonal antibody (STJ11105643) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 30  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.

Anti-CCL25 antibody (50-150) (STJ11105643)

SKU:
STJ11105643

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-C-C Motif Chemokine 25 (50-150) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CCL25
Gene ID: 6370
Uniprot ID: CCL25_HUMAN
Immunogen Region: 50-150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human CCL25 (NP_001381563.1).
Immunogen Sequence: RIQEVSGSCNLPAAIFYLPK RHRKVCGNPKSREVQRAMKL LDARNKVFAKLHHNTQTFQA GPHAVKKLSSGNSKLSSSKF SNPISSSKRNVSLLISANSG L
Tissue Specificity Specifically expressed by thymic dendritic cells. High levels in thymus and small intestine.
Function Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
Protein Name C-C Motif Chemokine 25
Chemokine Teck
Small-Inducible Cytokine A25
Thymus-Expressed Chemokine
Database Links Reactome: R-HSA-380108
Reactome: R-HSA-418594
Cellular Localisation Secreted
Alternative Antibody Names Anti-C-C Motif Chemokine 25 antibody
Anti-Chemokine Teck antibody
Anti-Small-Inducible Cytokine A25 antibody
Anti-Thymus-Expressed Chemokine antibody
Anti-CCL25 antibody
Anti-SCYA25 antibody
Anti-TECK antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance