Anti-CBX3 antibody (50-100 aa) (STJ13100978)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from aa region 50-100 of mouse CBX3 conjugated to an immunogenic carrier protein was used as the antigen.
General Information
| Short Description | Rabbit polyclonal anti-CBX3 (50-100 aa) for use in IHC and WB in Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB (confirmed by recombinant protein). A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20ยฐC for long term storage and refrigerated at 2-8ยฐC for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | Cbx3Uniprot ID=CBX3_MOUSE" |
| Immunogen | A synthetic peptide from aa region 50-100 of mouse CBX3 conjugated to an immunogenic carrier protein was used as the antigen. |
| Immunogen Region | 50-100 aa |
| Specificity | Specific for CBX3. |
Additional Info
| Function | Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Probably involved in the repression of many genes located in euchromatin, such as E2F1, MYC and CDC25A. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1. Mediates the recruitment of NIPBL to sites of DNA damage at double-strand breaks (DSBs). |
| Protein Name | Chromobox Protein Homolog 3Heterochromatin Protein 1 Homolog GammaHp1 GammaM32Modifier 2 Protein |
| Cellular Localisation | NucleusMay Be Associated With Microtubules And Mitotic Poles During Mitosis (Potential)Associates With Euchromatin And Is Largely Excluded From Constitutive Heterochromatin |
| Alternative Antibody Names | Anti-Chromobox Protein Homolog 3 antibodyAnti-Heterochromatin Protein 1 Homolog Gamma antibodyAnti-Hp1 Gamma antibodyAnti-M32 antibodyAnti-Modifier 2 Protein antibodyAnti-Cbx3 antibody |
Information sourced from Uniprot.org