• Immunofluorescence analysis of HeLa cells using CBS Rabbit monoclonal antibody (STJ11101369) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using CBS Rabbit monoclonal antibody (STJ11101369) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat lung using CBS Rabbit monoclonal antibody (STJ11101369) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using CBS Rabbit monoclonal antibody (STJ11101369) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using CBS Rabbit monoclonal antibody (STJ11101369) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CBS antibody (400-551) [S9MR] (STJ11101369)

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STJ11101369

£146.50 - £642.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:4000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CBS
Gene ID: 102724560/875
Uniprot ID: CBS_HUMAN
Immunogen Region: 400-551
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 400-551 of human CBS (P35520).
Immunogen Sequence: EDLTEKKPWWWHLRVQELGL SAPLTVLPTITCGHTIEILR EKGFDQAPVVDEAGVILGMV TLGNMLSSLLAGKVQPSDQV GKVIYKQFKQIRLTDTLGRL SHILEMDHFALVVHEQIQYH STGKSSQRQMVFGVVTAIDL LNFVAAQERDQK
Tissue Specificity In the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney.
Function Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine. Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons.
Protein Name Cystathionine Beta-Synthase
Beta-Thionase
Serine Sulfhydrase
Database Links Reactome: R-HSA-1614603
Reactome: R-HSA-2408508
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Cystathionine Beta-Synthase antibody
Anti-Beta-Thionase antibody
Anti-Serine Sulfhydrase antibody
Anti-CBS antibody

Information sourced from Uniprot.org

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