• Western blot analysis of extracts of various cell lines, using Caveolin-2 antibody (STJ11103373) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using Caveolin-2 rabbit polyclonal antibody (STJ11103373) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat lung using Caveolin-2 rabbit polyclonal antibody (STJ11103373) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH/3T3 cells using Caveolin-2 rabbit polyclonal antibody (STJ11103373) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-Caveolin-2 antibody (1-65) (STJ11103373)

SKU:
STJ11103373

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Caveolin-2 (1-65) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CAV2
Gene ID: 858
Uniprot ID: CAV2_HUMAN
Immunogen Region: 1-65
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-65 of human CAV2 (NP_001224.1).
Immunogen Sequence: MGLETEKADVQLFMDDDSYS HHSGLEYADPEKFADSDQDR DPHRLNSHLKLGFEDVIAEP VTTHS
Tissue Specificity Expressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts.
Post Translational Modifications Phosphorylated on serine and tyrosine residues. CAV1 promotes phosphorylation on Ser-23 which then targets the complex to the plasma membrane, lipid rafts and caveolae. Phosphorylation on Ser-36 appears to modulate mitosis in endothelial cells. Phosphorylation on both Tyr-19 and Tyr-27 is required for insulin-induced 'Ser-727' phosphorylation of STAT3 and its activation. Phosphorylation on Tyr-19 is required for insulin-induced phosphorylation of MAPK1 and DNA binding of STAT3. Tyrosine phosphorylation is induced by both EGF and insulin.
Function May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression.
Protein Name Caveolin-2
Database Links Reactome: R-HSA-9009391
Cellular Localisation Nucleus
Cytoplasm
Golgi Apparatus Membrane
Peripheral Membrane Protein
Cell Membrane
Membrane
Caveola
Potential Hairpin-Like Structure In The Membrane
Membrane Protein Of Caveolae
Tyr-19-Phosphorylated Form Is Enriched At Sites Of Cell-Cell Contact And Is Translocated To The Nucleus In Complex With Mapk1 In Response To Insulin
Tyr-27-Phosphorylated Form Is Located Both In The Cytoplasm And Plasma Membrane
Cav1-Mediated Ser-23-Phosphorylated Form Locates To The Plasma Membrane
Ser-36-Phosphorylated Form Resides In Intracellular Compartments
Alternative Antibody Names Anti-Caveolin-2 antibody
Anti-CAV2 antibody

Information sourced from Uniprot.org

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