• Western blot analysis of extracts of various cell lines, using Caveolin-3 antibody (STJ110389) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. _Lysates/proteins: 25ug per lane. _Blocking buffer: 3% non-fat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 60s.
  • Immunohistochemistry analysis of paraffin-embedded mouse lung using Caveolin-3 antibody (STJ110389) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human colon using Caveolin-3 antibody (STJ110389) at dilution of 1:200 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human colon using Caveolin-3 antibody (STJ110389) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse lung using Caveolin-3 antibody (STJ110389) at dilution of 1:200 (40x lens).
  • Western blot analysis of extracts of various cell lines, using Caveolin-3 antibody (STJ110389) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-CAV3 antibody (1-151) (STJ110389)

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STJ110389

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Caveolin-3 (1-151) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CAV3
Gene ID: 859
Uniprot ID: CAV3_HUMAN
Immunogen Region: 1-151
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-151 of human Caveolin-3 (NP_001225.1).
Immunogen Sequence: MMAEEHTDLEAQIVKDIHCK EIDLVNRDPKNINEDIVKVD FEDVIAEPVGTYSFDGVWKV SYTTFTVSKYWCYRLLSTLL GVPLALLWGFLFACISFCHI WAVVPCIKSYLIEIQCISHI YSLCIRTFCNPLFAALGQVC SSIKVVLRKEV
Tissue Specificity Expressed predominantly in muscle.
Post Translational Modifications Sumoylation with SUMO3 by PIAS4 may reduce agonist-induced internalization and desensitization of adrenergic receptor ABRD2.
Function May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. May also regulate voltage-gated potassium channels. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress. Mediates the recruitment of CAVIN2 and CAVIN3 proteins to the caveolae.
Protein Name Caveolin-3
M-Caveolin
Database Links Reactome: R-HSA-445355
Cellular Localisation Golgi Apparatus Membrane
Peripheral Membrane Protein
Cell Membrane
Membrane
Caveola
Sarcolemma
Potential Hairpin-Like Structure In The Membrane
Membrane Protein Of Caveolae
Alternative Antibody Names Anti-Caveolin-3 antibody
Anti-M-Caveolin antibody
Anti-CAV3 antibody

Information sourced from Uniprot.org

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