• Western blot analysis of extracts of various cell lines, using Caspase-9 antibody (STJ113147) at 1:1000 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37 °C for 5 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using Caspase-9 antibody (STJ113147) at 1:1000 dilution. L929 cells were treated by staurosporine (1 uM) for 3 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CASP9 antibody (250-350) (STJ113147)

SKU:
STJ113147

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Caspase-9 (250-350) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CASP9
Gene ID: 842
Uniprot ID: CASP9_HUMAN
Immunogen Region: 250-350
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 250-350 of human Caspase-9 (NP_001220.2).
Immunogen Sequence: VYGTDGCPVSVEKIVNIFNG TSCPSLGGKPKLFFIQACGG EQKDHGFEVASTSPEDESPG SNPEPDATPFQEGLRTFDQL DAISSLPTPSDIFVSYSTFP G
Tissue Specificity Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
Post Translational Modifications Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events. Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation. Phosphorylation on Tyr-153 by ABL1/c-Abl.occurs in the response of cells to DNA damage. (Microbial infection) ADP-riboxanation by C.violaceum CopC blocks CASP9 processing, preventing CASP9 activation and ability to mediate intrinsic apoptosis.
Function Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates effector caspases caspase-3 (CASP3) or caspase-7 (CASP7). Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2: Lacks activity is an dominant-negative inhibitor of caspase-9.
Protein Name Caspase-9
Casp-9
Apoptotic Protease Mch-6
Apoptotic Protease-Activating Factor 3
Apaf-3
Ice-Like Apoptotic Protease 6
Ice-Lap6 Cleaved Into - Caspase-9 Subunit P35 - Caspase-9 Subunit P10
Database Links Reactome: R-HSA-111458
Reactome: R-HSA-111459
Reactome: R-HSA-111463
Reactome: R-HSA-111464
Reactome: R-HSA-168638
Reactome: R-HSA-198323
Reactome: R-HSA-418889
Reactome: R-HSA-5674400
Reactome: R-HSA-9627069
Alternative Antibody Names Anti-Caspase-9 antibody
Anti-Casp-9 antibody
Anti-Apoptotic Protease Mch-6 antibody
Anti-Apoptotic Protease-Activating Factor 3 antibody
Anti-Apaf-3 antibody
Anti-Ice-Like Apoptotic Protease 6 antibody
Anti-Ice-Lap6 Cleaved Into - Caspase-9 Subunit P35 - Caspase-9 Subunit P10 antibody
Anti-CASP9 antibody
Anti-MCH6 antibody

Information sourced from Uniprot.org

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