| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | Casp8 |
| Gene ID: | 12370 |
| Uniprot ID: | CASP8_MOUSE |
| Immunogen Region: | 4-480 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 4-480 of mouse Caspase 8 (NP_033942.1). |
| Immunogen Sequence: | QSCLYAIAEELGSEDLAALK FLCLDYIPHKKQETIEDAQK LFLRLREKGMLEEGNLSFLK ELLFHISRWDLLVNFLDCNR EEMVRELRDPDNAQISPYRV MLFKLSEEVSELELRSFKFL LNNEIPKCKLEDDLSLLEIF VEMEKRTMLAENNLETLKSI CDQVNKSLLGKIEDYERSST ERRMSLEGREELPPSVLDEM SLKMAELCDSPREQDSESRT SDKVYQMKNKPRGYCLIIN |
| Tissue Specificity | Expressed in a wide variety of tissues. Highest expression in spleen, thymus, lung, liver and kidney. Lower expression in heart, brain, testis and skeletal muscle. |
| Post Translational Modifications | Generation of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events. (Microbial infection) Proteolytically cleaved by the cowpox virus CRMA death inhibitory protein. Phosphorylation on Ser-389 during mitosis by CDK1 inhibits activation by proteolysis and prevents apoptosis. This phosphorylation occurs in cancer cell lines, as well as in primary breast tissues and lymphocytes. |
| Function | Thiol protease that plays a key role in programmed cell death by acting as a molecular switch for apoptosis, necroptosis and pyroptosis, and is required to prevent tissue damage during embryonic development and adulthood. Initiator protease that induces extrinsic apoptosis by mediating cleavage and activation of effector caspases responsible for FAS/CD95-mediated and TNFRSF1A-induced cell death. Cleaves and activates effector caspases CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. Binding to the adapter molecule FADD recruits it to either receptor FAS/CD95 or TNFRSF1A. The resulting aggregate called the death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. In addition to extrinsic apoptosis, also acts as a negative regulator of necroptosis: acts by cleaving RIPK1 at 'Asp-325', which is crucial to inhibit RIPK1 kinase activity, limiting TNF-induced apoptosis, necroptosis and inflammatory response. Also able to initiate pyroptosis by mediating cleavage and activation of gasdermin-C and -D (GSDMC and GSDMD, respectively): gasdermin cleavage promotes release of the N-terminal moiety that binds to membranes and forms pores, triggering pyroptosis. Initiates pyroptosis following inactivation of MAP3K7/TAK1. Also acts as a regulator of innate immunity by mediating cleavage and inactivation of N4BP1 downstream of TLR3 or TLR4, thereby promoting cytokine production. May participate in the Granzyme B (GZMB) cell death pathways. Cleaves PARP1 and PARP2. |
| Protein Name | Caspase-8 Casp-8 Cleaved Into - Caspase-8 Subunit P18 - Caspase-8 Subunit P10 |
| Database Links | Reactome: R-MMU-111465 Reactome: -MMU-140534 Reactome: -MMU-168638 Reactome: -MMU-2562578 Reactome: -MMU-264870 Reactome: -MMU-3371378 Reactome: -MMU-5218900 Reactome: -MMU-5357905 Reactome: -MMU-5660668 Reactome: -MMU-5675482 Reactome: -MMU-69416 Reactome: -MMU-75108 Reactome: -MMU-75153 Reactome: -MMU-75157 Reactome: -MMU-75158 Reactome: -MMU-9758274 |
| Cellular Localisation | Cytoplasm Nucleus Translocates Into The Nucleus During Apoptosis |
| Alternative Antibody Names | Anti-Caspase-8 antibody Anti-Casp-8 Cleaved Into - Caspase-8 Subunit P18 - Caspase-8 Subunit P10 antibody Anti-Casp8 antibody |
Information sourced from Uniprot.org