• Flow cytometric analysis of Lovo cells using PRMT4 monoclonal antibody (green) and negative control (purple).
  • Immunohistochemistry analysis of paraffin-embedded breast cancer tissues (left) and ovarian cancer tissues (right) with DAB staining using PRMT4 monoclonal antibody.
  • Immunofluorescence analysis of Hela cells using PRMT4 monoclonal antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Western blot analysis using PRMT4 monoclonal antibody against MCF-7 (1) , HeLa (2) , NIH/3T3 (3) , HL-60 (4) , LNcap (5) , Jurkat (6) , PC-3 (7) , Cos7 (8) , and PC-12 (9) cell lysate.

Anti-CARM1 antibody [3H2] (STJ98337)

SKU:
STJ98337

£139.00 - £336.00
Current Stock:
Host: Mouse
Applications: WB/IHC/IF/FC/ELISA
Reactivity: Human/Rat/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Histone-arginine methyltransferase CARM1 is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry and ELISA research applications.
Clonality: Monoclonal
Clone ID: 3H2
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CARM1
Gene ID: 10498
Uniprot ID: CARM1_HUMAN
Specificity: PRMT4 Monoclonal Antibody detects endogenous levels of PRMT4 protein.
Immunogen: Purified recombinant fragment of human PRMT4 expressed in E. Coli.
Post Translational Modifications Auto-methylated on Arg-550. Methylation enhances transcription coactivator activity. Methylation is required for its role in the regulation of pre-mRNA alternative splicing. Phosphorylation at Ser-216 is strongly increased during mitosis, and decreases rapidly to a very low, basal level after entry into the G1 phase of the cell cycle. Phosphorylation at Ser-216 may promote location in the cytosol. Phosphorylation at Ser-216 interferes with S-adenosyl-L-methionine binding and strongly reduces methyltransferase activity. Ubiquitinated by E3 ubiquitin-protein ligase complex containing FBXO9 at Lys-227.leading to proteasomal degradation.
Function Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activates transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs. Acts as a transcriptional coactivator of ACACA/acetyl-CoA carboxylase by enriching H3R17 methylation at its promoter, thereby positively regulating fatty acid synthesis. Independently of its methyltransferase activity, involved in replication fork progression: promotes PARP1 recruitment to replication forks, leading to poly-ADP-ribosylation of chromatin at replication forks and reduced fork speed.
Protein Name Histone-Arginine Methyltransferase Carm1
Coactivator-Associated Arginine Methyltransferase 1
Protein Arginine N-Methyltransferase 4
Database Links Reactome: R-HSA-1368082
Reactome: R-HSA-1368108
Reactome: R-HSA-1989781
Reactome: R-HSA-2151201
Reactome: R-HSA-2426168
Reactome: R-HSA-3214858
Reactome: R-HSA-381340
Reactome: R-HSA-400206
Reactome: R-HSA-400253
Reactome: R-HSA-6804114
Reactome: R-HSA-9018519
Reactome: R-HSA-9707564
Reactome: R-HSA-9707616
Cellular Localisation Nucleus
Cytoplasm
Chromosome
Mainly Nuclear During The G1
S And G2 Phases Of The Cell Cycle
Cytoplasmic During Mitosis
After Breakup Of The Nuclear Membrane
Localizes To Replication Forks
Alternative Antibody Names Anti-Histone-Arginine Methyltransferase Carm1 antibody
Anti-Coactivator-Associated Arginine Methyltransferase 1 antibody
Anti-Protein Arginine N-Methyltransferase 4 antibody
Anti-CARM1 antibody
Anti-PRMT4 antibody

Information sourced from Uniprot.org

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