| Short Description : | Rabbit monoclonal Cardiac Troponin I (TNNI3) antibody for use in WB, IF-P and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IF-P/ELISA |
| Host: | Rabbit |
| Reactivity: | Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | SRM14456 |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Purification: | Affinity purification |
| Dilution Range: | WB, 1:1000-1:5000IF-P, 1:100-1:500ELISA, Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TNNI3 |
| Gene ID: | 7137 |
| Uniprot ID: | TNNI3_HUMAN |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Post Translational Modifications | Phosphorylated at Ser-42 and Ser-44 by PRKCE.phosphorylation increases myocardium contractile dysfunction. Phosphorylated at Ser-23 and Ser-24 by PRKD1.phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-31. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T). |
| Function | Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. |
| Protein Name | Troponin I - Cardiac MuscleCardiac Troponin I |
| Database Links | Reactome: R-HSA-390522Reactome: R-HSA-5578775 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Troponin I - Cardiac Muscle antibodyAnti-Cardiac Troponin I antibodyAnti-TNNI3 antibodyAnti-TNNC1 antibody |
Information sourced from Uniprot.org